DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast

Michael A. White, Monika Wierdl, Peter Detloff, Thomas D. Petes

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

In the yeast Saccharomyces cerevisiae, as in other eukaryotes, some regions of the genome have a much higher rate of meiotic recombination than others. We show below that the binding of the RAP1 protein to a site upstream of the HIS4 gene is necessary for a high rate of meiotic (but not mitotic) recombination at this locus. A mutation in the RAP1 binding site at HIS4 results in a decrease in recombination; overproduction of RAP1 causes an increase in recombination at HIS4 above wild-type levels.

Original languageEnglish (US)
Pages (from-to)9755-9759
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number21
DOIs
StatePublished - 1991

Keywords

  • Saccharomyces cerevisiae
  • gene conversion
  • recombination hotspot

ASJC Scopus subject areas

  • General

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