DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function

Paula L. Fischhaber, Lisa D. McDaniel, Errol C. Friedberg

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase κ (Polκ from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.

Original languageEnglish (US)
Pages (from-to)355-378
Number of pages24
JournalMethods in Enzymology
Volume408
DOIs
StatePublished - Aug 15 2006

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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