DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function

Paula L. Fischhaber, Lisa D. McDaniel, Errol C. Friedberg

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This chapter discusses experimental methods and protocols for the purification and preliminary characterization of DNA polymerases that are specialized for the replicative bypass (translesion DNA synthesis) of base or other types of DNA damage that typically arrest high-fidelity DNA synthesis, with particular emphasis on DNA polymerase κ (Polκ from mouse cells). It also describes some of the methods employed in the evaluation of mouse strains defective in genes that encode these enzymes.

Original languageEnglish (US)
Pages (from-to)355-378
Number of pages24
JournalMethods in Enzymology
Volume408
DOIs
StatePublished - 2006

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DNA-Directed DNA Polymerase
Purification
DNA
Enzymes
DNA Damage
Genes

ASJC Scopus subject areas

  • Biochemistry

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DNA Polymerases for Translesion DNA Synthesis : Enzyme Purification and Mouse Models for Studying Their Function. / Fischhaber, Paula L.; McDaniel, Lisa D.; Friedberg, Errol C.

In: Methods in Enzymology, Vol. 408, 2006, p. 355-378.

Research output: Contribution to journalArticle

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