Abstract
The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 441-447 |
| Number of pages | 7 |
| Journal | MGG Molecular & General Genetics |
| Volume | 181 |
| Issue number | 4 |
| DOIs | |
| State | Published - May 1981 |
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ASJC Scopus subject areas
- Genetics
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DNA sequence of a plasmid-encoded dihydrofolate reductase. / Swift, Galvin; McCarthy, Brian J.; Heffron, Fred.
In: MGG Molecular & General Genetics, Vol. 181, No. 4, 05.1981, p. 441-447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - DNA sequence of a plasmid-encoded dihydrofolate reductase
AU - Swift, Galvin
AU - McCarthy, Brian J.
AU - Heffron, Fred
PY - 1981/5
Y1 - 1981/5
N2 - The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.
AB - The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.
UR - http://www.scopus.com/inward/record.url?scp=0019493991&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019493991&partnerID=8YFLogxK
U2 - 10.1007/BF00428733
DO - 10.1007/BF00428733
M3 - Article
C2 - 7022127
AN - SCOPUS:0019493991
VL - 181
SP - 441
EP - 447
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 4
ER -