DNA sequence of a plasmid-encoded dihydrofolate reductase

Galvin Swift, Brian J. McCarthy, Fred Heffron

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The sequence of the methotrexate-resistant dihydrofolate reductase (DHFR) gene borne by the plasmid R-388 was determined. The gene was subcloned and mapped by an in vitro mutagenesis method involving insertion of synthetic oligonucleotide decamers encoding the BamHI recognition site. Sites of insertion that destroyed the methotrexate resistance fell in two regions separated by 300 bp within a 1.2 kb fragment. One of these regions encodes a 78 amino acid polypeptide homologous to another drug-resistant DHFR. The second region essential for DHFR expression appears to be the promoter of the DHFR gene.

Original languageEnglish (US)
Pages (from-to)441-447
Number of pages7
JournalMGG Molecular & General Genetics
Volume181
Issue number4
DOIs
StatePublished - May 1981

Fingerprint

Tetrahydrofolate Dehydrogenase
Plasmids
Methotrexate
R388
Genes
Oligonucleotides
Mutagenesis
Amino Acids
Peptides
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Genetics

Cite this

DNA sequence of a plasmid-encoded dihydrofolate reductase. / Swift, Galvin; McCarthy, Brian J.; Heffron, Fred.

In: MGG Molecular & General Genetics, Vol. 181, No. 4, 05.1981, p. 441-447.

Research output: Contribution to journalArticle

Swift, Galvin ; McCarthy, Brian J. ; Heffron, Fred. / DNA sequence of a plasmid-encoded dihydrofolate reductase. In: MGG Molecular & General Genetics. 1981 ; Vol. 181, No. 4. pp. 441-447.
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