DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster Abelson proto-oncogene homolog.

M. J. Henkemeyer, R. L. Bennett, F. B. Gertler, F. M. Hoffmann

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We report our molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a protein of 1,520 amino acids with strong sequence similarity to the human c-abl proto-oncogene beginning in the type lb 5' exon and extending through the region essential for tyrosine kinase activity. When the tyrosine kinase homologous region was expressed in Escherichia coli, phosphorylation of proteins on tyrosine residues was observed with an antiphosphotyrosine antibody. These results show that the abl gene is highly conserved through evolution and encodes a functional tyrosine protein kinase required for Drosophila development.

Original languageEnglish (US)
Pages (from-to)843-853
Number of pages11
JournalMolecular and Cellular Biology
Volume8
Issue number2
StatePublished - Feb 1988

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abl Genes
Proto-Oncogenes
Drosophila melanogaster
Protein-Tyrosine Kinases
Exons
Escherichia coli Proteins
Essential Genes
Drosophila
Tyrosine
Phosphorylation
Amino Acids
Mutation
Antibodies
DNA
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster Abelson proto-oncogene homolog. / Henkemeyer, M. J.; Bennett, R. L.; Gertler, F. B.; Hoffmann, F. M.

In: Molecular and Cellular Biology, Vol. 8, No. 2, 02.1988, p. 843-853.

Research output: Contribution to journalArticle

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