DNA sequence, structure, and tyrosine kinase activity of the Drosophila melanogaster Abelson proto-oncogene homolog.

M. J. Henkemeyer, R. L. Bennett, F. B. Gertler, F. M. Hoffmann

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

We report our molecular characterization of the Drosophila melanogaster Abelson gene (abl), a gene in which recessive loss-of-function mutations result in lethality at the pupal stage of development. This essential gene consists of 10 exons extending over 26 kilobase pairs of genomic DNA. The DNA sequence encodes a protein of 1,520 amino acids with strong sequence similarity to the human c-abl proto-oncogene beginning in the type lb 5' exon and extending through the region essential for tyrosine kinase activity. When the tyrosine kinase homologous region was expressed in Escherichia coli, phosphorylation of proteins on tyrosine residues was observed with an antiphosphotyrosine antibody. These results show that the abl gene is highly conserved through evolution and encodes a functional tyrosine protein kinase required for Drosophila development.

Original languageEnglish (US)
Pages (from-to)843-853
Number of pages11
JournalMolecular and cellular biology
Volume8
Issue number2
DOIs
StatePublished - Feb 1988

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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