DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase

Natalie M. Stano, Yong Joo Jeong, Ilker Donmez, Padmaja Tummalapalli, Mikhail K. Levin, Smita S. Patel

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Helicases are molecular motors that use the energy of nucleoside 5′-triphosphate (NTP) hydrolysis to translocate along a nucleic acid strand and catalyse reactions such as DNA unwinding. The ring-shaped helicase of bacteriophage T7 translocates along single-stranded (ss)DNA at a speed of 130 bases per second; however, T7 helicase slows down nearly tenfold when unwinding the strands of duplex DNA. Here, we report that T7 DNA polymerase, which is unable to catalyse strand displacement DNA synthesis by itself, can increase the unwinding rate to 114 base pairs per second, bringing the helicase up to similar speeds compared to its translocation along ssDNA. The helicase rate of stimulation depends upon the DNA synthesis rate and does not rely on specific interactions between T7 DNA polymerase and the carboxy-terminal residues of T7 helicase. Efficient duplex DNA synthesis is achieved only by the combined action of the helicase and polymerase. The strand displacement DNA synthesis by the DNA polymerase depends on the unwinding activity of the helicase, which provides ssDNA template. The rapid trapping of the ssDNA bases by the DNA synthesis activity of the polymerase in turn drives the helicase to move forward through duplex DNA at speeds similar to those observed along ssDNA.

Original languageEnglish (US)
Pages (from-to)370-373
Number of pages4
JournalNature
Volume435
Issue number7040
DOIs
StatePublished - May 19 2005

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DNA
DNA-Directed DNA Polymerase
Bacteriophage T7
Single-Stranded DNA
Nucleosides
Base Pairing
Nucleic Acids
Hydrolysis

ASJC Scopus subject areas

  • General

Cite this

Stano, N. M., Jeong, Y. J., Donmez, I., Tummalapalli, P., Levin, M. K., & Patel, S. S. (2005). DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase. Nature, 435(7040), 370-373. https://doi.org/10.1038/nature03615

DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase. / Stano, Natalie M.; Jeong, Yong Joo; Donmez, Ilker; Tummalapalli, Padmaja; Levin, Mikhail K.; Patel, Smita S.

In: Nature, Vol. 435, No. 7040, 19.05.2005, p. 370-373.

Research output: Contribution to journalArticle

Stano, NM, Jeong, YJ, Donmez, I, Tummalapalli, P, Levin, MK & Patel, SS 2005, 'DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase', Nature, vol. 435, no. 7040, pp. 370-373. https://doi.org/10.1038/nature03615
Stano NM, Jeong YJ, Donmez I, Tummalapalli P, Levin MK, Patel SS. DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase. Nature. 2005 May 19;435(7040):370-373. https://doi.org/10.1038/nature03615
Stano, Natalie M. ; Jeong, Yong Joo ; Donmez, Ilker ; Tummalapalli, Padmaja ; Levin, Mikhail K. ; Patel, Smita S. / DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase. In: Nature. 2005 ; Vol. 435, No. 7040. pp. 370-373.
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