DNA typing for class II HLA antigens with allele-specific or group-specific amplification. I. Typing for subsets of HLA-DR4

Xiaojiang Gao, Marcelo Fernandez-Vina, Wayne Shumway, Peter Stastny

Research output: Contribution to journalArticle

108 Scopus citations

Abstract

DNA sequences that distincuish the subsets of HLA-DR4 are also found on several other alleles. This makes typing of beterozygotes with oligonucleotide probes quite impractical. We have therefore developed a procedure in which, in a first step, DNA of the genes to be analyzed is amplified selectively, using group-specific primers. In the case of DR4-DRB1, a primer matching codons 5 to 13 when used in the polymerase chain reaction resulted in products that were entirely suitable for typing for the DR4 subsets. Using appropriate probes, eight distinct subsets were identified. However, only six of them were represented in a normal North American Caucasian panel. In conjunction with a method for rapid DNA extraction, the procedure offers a simple, highly specific and reproducible method for determining subtypes of HLA-DR4 that at present cannot be recognized by serologic methods.

Original languageEnglish (US)
Pages (from-to)40-50
Number of pages11
JournalHuman Immunology
Volume27
Issue number1
DOIs
StatePublished - Jan 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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