Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum

L. Liscum, J. Finer-Moore, R. M. Stroud, K. L. Luskey, M. S. Brown, J. L. Goldstein

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

We present and evaluate a model for the secondary structure and membrane orientation of 3-hydroxy-3-methylglutaryl coenzyme A reductase, the glycoprotein of the endoplasmic reticulum that controls the rate of cholesterol biosynthesis. This model is derived from proteolysis experiments that separate the 97-kilodalton enzyme into two domains, an NH2-terminal membrane-bound domain of 339 residues and a COOH-terminal water-soluble domain of 548 residues that projects into the cytoplasm and contains the catalytic site. These domains were identified by reaction with antibodies against synthetic peptides corresponding to specific regions in the molecule. Computer modeling of the reductase structure, based on he amino acid sequence as determined by molecular cloning, predicts that the NH2-terminal domain contains 7 membrane-spanning regions. Analysis of the gene structure reveals that each proposed membrane-spanning region is encoded in a separate exon and is separated from the adjacent membrane-spanning region by an intron. The COOH-terminal domain of the reductase is predicted to contain two β-structures flanked by a series of amphipathic helices, which together may constitute the active site. The NH2-terminal membrane-bound domain of the reductase bears some resemblance to rhodopsin, the photoreceptor protein of retinal rod disks and the only other intracellular glycoprotein whose amino acid sequence is known.

Original languageEnglish (US)
Pages (from-to)522-530
Number of pages9
JournalJournal of Biological Chemistry
Volume260
Issue number1
StatePublished - 1985

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Endoplasmic Reticulum
Glycoproteins
Oxidoreductases
Membranes
Amino Acid Sequence
Catalytic Domain
Proteolysis
Retinal Rod Photoreceptor Cells
Amino Acids
Rhodopsin
Cloning
Biosynthesis
Molecular Cloning
Introns
3-hydroxy-3-methylglutaryl-coenzyme A
Exons
Cytoplasm
Genes
Cholesterol
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum. / Liscum, L.; Finer-Moore, J.; Stroud, R. M.; Luskey, K. L.; Brown, M. S.; Goldstein, J. L.

In: Journal of Biological Chemistry, Vol. 260, No. 1, 1985, p. 522-530.

Research output: Contribution to journalArticle

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AU - Brown, M. S.

AU - Goldstein, J. L.

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