Double isotype production by a neoplastic B cell line. I. Cellular and biochemical characterization of a variant of BCL1 that expresses and secretes both IgM and IgG1

Y. W. Chen, C. J. Word, S. Jones, J. W. Uhr, P. W. Tucker, E. S. Vitetta

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have subcloned the in vitro-adapted murine B cell leukemia, BCL1.B1, to obtain a variant that expresses both IgM and IgG1. By fluorescence analysis, radioiodination, and immunoprecipitation of cell surface Ig, and by RIA of medium from limiting dilution cultures, we have shown that: (1) all the cells express and secrete both isotypes. The heavy chains of both IgG1 and IgM have the apparent molecular weights of membrane μ and γ1 chains; (b) both isotypes bear the same idiotype as determined by immunoprecipitation with antiidiotypic antibody, and both use the same VDJ rearrangement as shown by Southern blotting; and (c) the cells express the membrane and secreted forms of mRNA for both μ and γ1 but not γ2b or γ3. Taken together, the data suggest that all the cells are synthesizing, expressing on their surface, and secreting two isotypes that use the same VDJ rearrangement in the DNA and express the same serologically-defined idiotype. The molecular basis responsible for the production of the two isotypes in a single cell is the subject of the accompanying paper.

Original languageEnglish (US)
Pages (from-to)548-561
Number of pages14
JournalJournal of Experimental Medicine
Volume164
Issue number2
StatePublished - 1986

Fingerprint

Immunoglobulin M
B-Lymphocytes
Immunoglobulin G
Cell Line
Immunoprecipitation
B-Cell Leukemia
Gene Rearrangement
Southern Blotting
Molecular Weight
Fluorescence
Cell Membrane
Messenger RNA
Membranes
Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Double isotype production by a neoplastic B cell line. I. Cellular and biochemical characterization of a variant of BCL1 that expresses and secretes both IgM and IgG1. / Chen, Y. W.; Word, C. J.; Jones, S.; Uhr, J. W.; Tucker, P. W.; Vitetta, E. S.

In: Journal of Experimental Medicine, Vol. 164, No. 2, 1986, p. 548-561.

Research output: Contribution to journalArticle

@article{388a5a2e4c1c440495011bc9f5f43783,
title = "Double isotype production by a neoplastic B cell line. I. Cellular and biochemical characterization of a variant of BCL1 that expresses and secretes both IgM and IgG1",
abstract = "We have subcloned the in vitro-adapted murine B cell leukemia, BCL1.B1, to obtain a variant that expresses both IgM and IgG1. By fluorescence analysis, radioiodination, and immunoprecipitation of cell surface Ig, and by RIA of medium from limiting dilution cultures, we have shown that: (1) all the cells express and secrete both isotypes. The heavy chains of both IgG1 and IgM have the apparent molecular weights of membrane μ and γ1 chains; (b) both isotypes bear the same idiotype as determined by immunoprecipitation with antiidiotypic antibody, and both use the same VDJ rearrangement as shown by Southern blotting; and (c) the cells express the membrane and secreted forms of mRNA for both μ and γ1 but not γ2b or γ3. Taken together, the data suggest that all the cells are synthesizing, expressing on their surface, and secreting two isotypes that use the same VDJ rearrangement in the DNA and express the same serologically-defined idiotype. The molecular basis responsible for the production of the two isotypes in a single cell is the subject of the accompanying paper.",
author = "Chen, {Y. W.} and Word, {C. J.} and S. Jones and Uhr, {J. W.} and Tucker, {P. W.} and Vitetta, {E. S.}",
year = "1986",
language = "English (US)",
volume = "164",
pages = "548--561",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "2",

}

TY - JOUR

T1 - Double isotype production by a neoplastic B cell line. I. Cellular and biochemical characterization of a variant of BCL1 that expresses and secretes both IgM and IgG1

AU - Chen, Y. W.

AU - Word, C. J.

AU - Jones, S.

AU - Uhr, J. W.

AU - Tucker, P. W.

AU - Vitetta, E. S.

PY - 1986

Y1 - 1986

N2 - We have subcloned the in vitro-adapted murine B cell leukemia, BCL1.B1, to obtain a variant that expresses both IgM and IgG1. By fluorescence analysis, radioiodination, and immunoprecipitation of cell surface Ig, and by RIA of medium from limiting dilution cultures, we have shown that: (1) all the cells express and secrete both isotypes. The heavy chains of both IgG1 and IgM have the apparent molecular weights of membrane μ and γ1 chains; (b) both isotypes bear the same idiotype as determined by immunoprecipitation with antiidiotypic antibody, and both use the same VDJ rearrangement as shown by Southern blotting; and (c) the cells express the membrane and secreted forms of mRNA for both μ and γ1 but not γ2b or γ3. Taken together, the data suggest that all the cells are synthesizing, expressing on their surface, and secreting two isotypes that use the same VDJ rearrangement in the DNA and express the same serologically-defined idiotype. The molecular basis responsible for the production of the two isotypes in a single cell is the subject of the accompanying paper.

AB - We have subcloned the in vitro-adapted murine B cell leukemia, BCL1.B1, to obtain a variant that expresses both IgM and IgG1. By fluorescence analysis, radioiodination, and immunoprecipitation of cell surface Ig, and by RIA of medium from limiting dilution cultures, we have shown that: (1) all the cells express and secrete both isotypes. The heavy chains of both IgG1 and IgM have the apparent molecular weights of membrane μ and γ1 chains; (b) both isotypes bear the same idiotype as determined by immunoprecipitation with antiidiotypic antibody, and both use the same VDJ rearrangement as shown by Southern blotting; and (c) the cells express the membrane and secreted forms of mRNA for both μ and γ1 but not γ2b or γ3. Taken together, the data suggest that all the cells are synthesizing, expressing on their surface, and secreting two isotypes that use the same VDJ rearrangement in the DNA and express the same serologically-defined idiotype. The molecular basis responsible for the production of the two isotypes in a single cell is the subject of the accompanying paper.

UR - http://www.scopus.com/inward/record.url?scp=0022535093&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022535093&partnerID=8YFLogxK

M3 - Article

C2 - 2425035

AN - SCOPUS:0022535093

VL - 164

SP - 548

EP - 561

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 2

ER -