Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication

Stephanie Blachon, Jayachandran Gopalakrishnan, Yoshihiro Omori, Andrey Polyanovsky, Allen Church, Daniela Nicastro, Jarema Malicki, Tomer Avidor-Reiss

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Abstract

The centriole is the core structure of centrosome and cilium. Failure to restrict centriole duplication to once per cell cycle has serious consequences and is commonly observed in cancer. Despite its medical importance, the mechanism of centriole formation is poorly understood. Asl was previously reported to be a centrosomal protein essential for centrosome function. Here we identify mecD, a severe loss-of-function allele of the asl gene, and demonstrate that it is required for centriole and cilia formation. Similarly, Cep152, the Asl ortholog in vertebrates, is essential for cilia formation and its function can be partially rescued by the Drosophila Asl. The study of Asl localization suggests that it is closely associated with the centriole wall, but is not part of the centriole structure. By analyzing the biogenesis of centrosomes in cells depleted of Asl, we found that, while pericentriolar material (PCM) function is mildly affected, Asl is essential for daughter centriole formation. The clear absence of several centriolar markers in mecD mutants suggests that Asl is critical early in centriole duplication.

Original languageEnglish (US)
Pages (from-to)2081-2094
Number of pages14
JournalGenetics
Volume180
Issue number4
DOIs
StatePublished - Dec 1 2008

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Centrioles
Drosophila
Vertebrates
Centrosome
Cilia
Cell Cycle
Alleles

ASJC Scopus subject areas

  • Genetics

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Blachon, S., Gopalakrishnan, J., Omori, Y., Polyanovsky, A., Church, A., Nicastro, D., ... Avidor-Reiss, T. (2008). Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication. Genetics, 180(4), 2081-2094. https://doi.org/10.1534/genetics.108.095141

Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication. / Blachon, Stephanie; Gopalakrishnan, Jayachandran; Omori, Yoshihiro; Polyanovsky, Andrey; Church, Allen; Nicastro, Daniela; Malicki, Jarema; Avidor-Reiss, Tomer.

In: Genetics, Vol. 180, No. 4, 01.12.2008, p. 2081-2094.

Research output: Contribution to journalArticle

Blachon, S, Gopalakrishnan, J, Omori, Y, Polyanovsky, A, Church, A, Nicastro, D, Malicki, J & Avidor-Reiss, T 2008, 'Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication', Genetics, vol. 180, no. 4, pp. 2081-2094. https://doi.org/10.1534/genetics.108.095141
Blachon, Stephanie ; Gopalakrishnan, Jayachandran ; Omori, Yoshihiro ; Polyanovsky, Andrey ; Church, Allen ; Nicastro, Daniela ; Malicki, Jarema ; Avidor-Reiss, Tomer. / Drosophila asterless and vertebrate Cep152 are orthologs essential for centriole duplication. In: Genetics. 2008 ; Vol. 180, No. 4. pp. 2081-2094.
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