Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets

Ronald S. Flannagan, Rene E. Harrison, Christopher M. Yip, Khuloud Jaqaman, Sergio Grinstein

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Binding of ligands by immunoreceptors is thought to be a passive, stochastic process. Contrary to this notion, we found that binding of IgG-opsonized particles by Fcγ receptors was inhibited in macrophages, dendritic and microglial cells by agents that interfere with actin assembly or disassembly. Changes in the lateral mobility of the receptors - assessed by single-particle tracking - or in the microelasticity of the membrane - determined by atomic-force microscopy - could not account for the effects of actin disruption on particle binding. Instead, we found that the macrophages contact their targets by actively extending actin-rich structures. Formation of these protrusions is driven by Rac and requires phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Capture of C3bi-opsonized as well as unopsonized targets by macrophages was also dependent on actin. Thus, phagocytes continuously probe their environment for foreign particles in a manner akin to the constitutive sampling of the fluid milieu by dendritic cells. Active probing by phagocytes is most important when confronted by scarcely opsonized and/or highly mobile targets.

Original languageEnglish (US)
Pages (from-to)1205-1218
Number of pages14
JournalJournal of Cell Biology
Volume191
Issue number6
DOIs
StatePublished - Dec 13 2010

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Actins
Macrophages
Phagocytes
Dendritic Cells
Complement C3b
Stochastic Processes
Fc Receptors
Atomic Force Microscopy
Phosphatidylinositols
Immunoglobulin G
Ligands
Membranes

ASJC Scopus subject areas

  • Cell Biology

Cite this

Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets. / Flannagan, Ronald S.; Harrison, Rene E.; Yip, Christopher M.; Jaqaman, Khuloud; Grinstein, Sergio.

In: Journal of Cell Biology, Vol. 191, No. 6, 13.12.2010, p. 1205-1218.

Research output: Contribution to journalArticle

Flannagan, Ronald S. ; Harrison, Rene E. ; Yip, Christopher M. ; Jaqaman, Khuloud ; Grinstein, Sergio. / Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets. In: Journal of Cell Biology. 2010 ; Vol. 191, No. 6. pp. 1205-1218.
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