Dynamin is membrane-active: Lipid insertion is induced by phosphoinositides and phosphatidic acid

K. N J Burger, R. A. Demel, S. L. Schmid, B. De Kruijff

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Dynamin is a large GTPase involved in the regulation of membrane constriction and fission during receptor-mediated endocytosis. Dynamin contains a pleckstrin-homology domain which is essential for endocytosis and which binds to anionic phospholipids. Here, we show for the first time that dynamin is a membrane-active molecule capable of penetrating into the acyl chain region of membrane lipids. Lipid penetration is strongly stimulated by phosphatidic acid (PA), phosphatidylinositol 4-phosphate, and phosphatidylinositol 4,5-bisphosphate. Though binding is more efficient in the presence of the phosphoinositides, a much larger part of the dynamin molecule penetrates into PA-containing mixed-lipid systems. Thus, local lipid metabolism will dramatically influence dynamin-lipid interactions, and dynamin-lipid interactions are likely to play an important role in dynamin-dependent endocytosis. Our data suggest that dynamin is directly involved in membrane destabilization, a prerequisite to membrane fission.

Original languageEnglish (US)
Pages (from-to)12485-12493
Number of pages9
JournalBiochemistry
Volume39
Issue number40
DOIs
StatePublished - Oct 10 2000

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Dynamins
Phosphatidic Acids
Membrane Lipids
Phosphatidylinositols
Membranes
Lipids
Endocytosis
Molecules
GTP Phosphohydrolases
Lipid Metabolism
Constriction
Phospholipids

ASJC Scopus subject areas

  • Biochemistry

Cite this

Dynamin is membrane-active : Lipid insertion is induced by phosphoinositides and phosphatidic acid. / Burger, K. N J; Demel, R. A.; Schmid, S. L.; De Kruijff, B.

In: Biochemistry, Vol. 39, No. 40, 10.10.2000, p. 12485-12493.

Research output: Contribution to journalArticle

Burger, K. N J ; Demel, R. A. ; Schmid, S. L. ; De Kruijff, B. / Dynamin is membrane-active : Lipid insertion is induced by phosphoinositides and phosphatidic acid. In: Biochemistry. 2000 ; Vol. 39, No. 40. pp. 12485-12493.
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