EAPII interacts with ETS1 and modulates its transcriptional function

Huiping Pei, John S. Yordy, Qixin Leng, Qinghua Zhao, Dennis K. Watson, Runzhao Li

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Ets proteins constitute a family of conserved sequence-specific DNA-binding proteins and function as transcription factors. ETS1 plays important roles in differentiation, lymphoid cell development, invasiveness and angiogenesis. Such diverse roles of ETS1 are likely to be dependent on its associated proteins. A yeast two-hybrid screen was conducted and here we describe a novel ETS1 interacting protein designated as ETS1-associated protein II (EAPII). EAPII protein interacts with ETS1 and other Ets proteins (ETS2 and FLI1) both in vitro and in vivo. Indirect immunofluorescence demonstrated that EAPII is predominately localized to the nucleus of mammalian cells. EAPII negatively modulates ETS1 transcriptional activity and attenuates synergistic transactivation by ETS1 and AP-1. Significantly, re-expression of EAPII inhibits the migration of epithelial cancer cells, but does not affect cell viability. Therefore, EAPII is a novel ETS1 modulator that regulates specific aspects of the ETS1 functions.

Original languageEnglish (US)
Pages (from-to)2699-2709
Number of pages11
JournalOncogene
Volume22
Issue number18
DOIs
StatePublished - May 8 2003

Fingerprint

Proteins
Conserved Sequence
Transcription Factor AP-1
DNA-Binding Proteins
Indirect Fluorescent Antibody Technique
Cell Nucleus
Transcriptional Activation
Cell Survival
Transcription Factors
Yeasts
Epithelial Cells
Lymphocytes
Neoplasms

Keywords

  • EAPII
  • ETS1
  • Migration
  • Transcriptional control

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Pei, H., Yordy, J. S., Leng, Q., Zhao, Q., Watson, D. K., & Li, R. (2003). EAPII interacts with ETS1 and modulates its transcriptional function. Oncogene, 22(18), 2699-2709. https://doi.org/10.1038/sj.onc.1206374

EAPII interacts with ETS1 and modulates its transcriptional function. / Pei, Huiping; Yordy, John S.; Leng, Qixin; Zhao, Qinghua; Watson, Dennis K.; Li, Runzhao.

In: Oncogene, Vol. 22, No. 18, 08.05.2003, p. 2699-2709.

Research output: Contribution to journalArticle

Pei, H, Yordy, JS, Leng, Q, Zhao, Q, Watson, DK & Li, R 2003, 'EAPII interacts with ETS1 and modulates its transcriptional function', Oncogene, vol. 22, no. 18, pp. 2699-2709. https://doi.org/10.1038/sj.onc.1206374
Pei H, Yordy JS, Leng Q, Zhao Q, Watson DK, Li R. EAPII interacts with ETS1 and modulates its transcriptional function. Oncogene. 2003 May 8;22(18):2699-2709. https://doi.org/10.1038/sj.onc.1206374
Pei, Huiping ; Yordy, John S. ; Leng, Qixin ; Zhao, Qinghua ; Watson, Dennis K. ; Li, Runzhao. / EAPII interacts with ETS1 and modulates its transcriptional function. In: Oncogene. 2003 ; Vol. 22, No. 18. pp. 2699-2709.
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