Effect of insulin and transferrin in the maintenance of the activated state of the T-lymphocyte induced by allo-antigen

J. H. Helderman, R. Gruchalla, L. E. Edwards

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Insulin is an immuno-modulating molecule enhancing cytotoxic T-cell function and supporting intermediary metabolism in activated lymphocytes. Insulin also maintains the activated state of mitogen-stimulated lymphocytes. Because of the importance of allo-antigen response of diabetic patients for infections and transplant immunity, we explored the role of insulin in the allo-activated state of lymphocytes. Allo-stimulation was provided in one-way mixed lymphocyte reactions, with Balb/C mouse lymph node cells responding to irradiated spleen cells of C57BL/6 mice, in culture under conditions which permitted evaluation of hormonal modulation. Mixed lymphocyte reactions established in fetal calf serum, insulin-depleted by passage over antibody affinity columns, were of equal magnitude to those in insulin-replete serum. A weak but positive mixed lymphocyte reaction can be effected with an artificial serum substitute containing no additional hormone. Provision of insulin (10 nmol/l) restored a full mixed lymphocyte reaction response which was mimicked by addition of transferrin. The insulin-transferrin combination was not additive. In conclusion, insulin and transferrin maintain the allo-activated state of the mouse lymphocyte, transferrin alone being sufficient when full antigenic stimulation is provided. If the same applies to human lymphocytes, insulin-deficient diabetic patients will respond to full allo-antigen challenge, and so should be fully capable of mounting a transplant immune response.

Original languageEnglish (US)
Pages (from-to)99-101
Number of pages3
JournalDiabetologia
Volume27
Issue number1 Supplement
DOIs
StatePublished - Jul 1984

Fingerprint

Transferrin
Maintenance
Insulin
T-Lymphocytes
Antigens
Mixed Lymphocyte Culture Test
Lymphocytes
Serum
Transplants
Antibody Affinity
Inbred C57BL Mouse
Mitogens
Immunity
Spleen
Lymph Nodes
Hormones
Infection

Keywords

  • allo-antigen mouse
  • insulin
  • T-lymphocyte
  • Transferrin

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Effect of insulin and transferrin in the maintenance of the activated state of the T-lymphocyte induced by allo-antigen. / Helderman, J. H.; Gruchalla, R.; Edwards, L. E.

In: Diabetologia, Vol. 27, No. 1 Supplement, 07.1984, p. 99-101.

Research output: Contribution to journalArticle

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