Effects of 20-HETE and 19(S)-HETE on rabbit proximal straight tubule volume transport

Raymond P Quigley, Michel G Baum, Komandla Malla Reddy, James C. Griener, J R Falck

Research output: Contribution to journalArticle

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Abstract

The kidney has the highest abundance of cytochrome P-450 of all extrahepatic organs. Within the kidney, the highest concentration of cytochrome P-450 is found in the proximal tubule. Whether 20- or 19(S)- hydroxyeicosatetraenoic acid (HETE), the major P-450 metabolites of arachidonic acid in the proximal tubule, affect transport in this segment has not been previously investigated. We examined the direct effects of 20- and 19(S)-HETE on volume absorption (J(v)) in the rabbit proximal straight tubule (PST). Production of 20-HETE by rabbit PST was demonstrated by incubating microdissected tubules with [3H]arachidonic acid and separating the lipid extract by HPLC. There was significant conversion of [3H]arachidonic acid to 20-HETE in control tubules that was inhibited by 10-5 M N-methylsulfonyl- 12,12-dibromododec-11-enamide (DDMS). Addition of exogenous 20-HETE had no effect on PST volume transport. However, inhibition of endogenous production of 20-HETE using DDMS stimulated transport. In the presence of DDMS, 20-HETE inhibited PST J(v). 19(S)-HETE in the bathing solution stimulated PST J(v) alone and in the presence of DDMS. Thus ω- and ω-1-hydroxylase products of arachidonic acid have direct effects on PST transport. Endogenous production of 20-HETE may play a role in tonic suppression of transport and may therefore be an endogenous regulator of transport in the proximal tubule.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume278
Issue number6 47-6
StatePublished - Jun 2000

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Rabbits
Arachidonic Acid
Cytochrome P-450 Enzyme System
Kidney
Mixed Function Oxygenases
19-hydroxy-5,8,11,14-eicosatetraenoic acid
20-hydroxy-5,8,11,14-eicosatetraenoic acid
High Pressure Liquid Chromatography
DDMS
Lipids

Keywords

  • ω- hydroxylase
  • Cytochrome P-450
  • Hydroxyeicosatetraenoic acids
  • Hypertension
  • In vitro microperfusion
  • Sodium-potassium- adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Effects of 20-HETE and 19(S)-HETE on rabbit proximal straight tubule volume transport. / Quigley, Raymond P; Baum, Michel G; Reddy, Komandla Malla; Griener, James C.; Falck, J R.

In: American Journal of Physiology - Renal Physiology, Vol. 278, No. 6 47-6, 06.2000.

Research output: Contribution to journalArticle

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