Effects of caffeine on hippocampal pyramidal cells in vitro

R. W. Greene, H. L. Haas, A. Hermann

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

1. The effects of caffeine on the electrophysiological properties of CA1 pyramidal neurones were investigated in the rat hippocampal slice preparation in vitro. 2. A concentration-dependent increase in both the extracellularly recorded excitatory postsynaptic potential (e.p.s.p.) and the population spike resulting from stimulation of the stratum radiatum could be evoked by caffeine with a threshold concentration of 10 μM. 3. Intracellular recordings demonstrate a caffeine-evoked decrease in resting membrane potential, an increase in input resistance, a reduction of the long afterhyperpolarization (a.h.p.) and a decrease in accommodation. 4. The interaction between caffeine and adenosine was investigated on the extracellularly recorded e.p.s.p. The maximal response evoked by caffeine was increased in the presence of adenosine and the adenosine concentration-response curve was shifted to the right in a parallel fashion in the presence of caffeine. 5. It is suggested that the effects of the caffeine on hippocampal neurones may be mediated by a decrease of one or more potassium conductance(s), and that adenosine and caffeine may compete for the same electrophysiological active receptor site on these cells.

Original languageEnglish (US)
Pages (from-to)163-169
Number of pages7
JournalBritish Journal of Pharmacology
Volume85
Issue number1
StatePublished - 1985

Fingerprint

Pyramidal Cells
Caffeine
Adenosine
Excitatory Postsynaptic Potentials
In Vitro Techniques
Membrane Potentials
Catalytic Domain
Potassium
Neurons

ASJC Scopus subject areas

  • Pharmacology

Cite this

Effects of caffeine on hippocampal pyramidal cells in vitro. / Greene, R. W.; Haas, H. L.; Hermann, A.

In: British Journal of Pharmacology, Vol. 85, No. 1, 1985, p. 163-169.

Research output: Contribution to journalArticle

Greene, R. W. ; Haas, H. L. ; Hermann, A. / Effects of caffeine on hippocampal pyramidal cells in vitro. In: British Journal of Pharmacology. 1985 ; Vol. 85, No. 1. pp. 163-169.
@article{e918b16a311c4bb5b7741602d3ef82ad,
title = "Effects of caffeine on hippocampal pyramidal cells in vitro",
abstract = "1. The effects of caffeine on the electrophysiological properties of CA1 pyramidal neurones were investigated in the rat hippocampal slice preparation in vitro. 2. A concentration-dependent increase in both the extracellularly recorded excitatory postsynaptic potential (e.p.s.p.) and the population spike resulting from stimulation of the stratum radiatum could be evoked by caffeine with a threshold concentration of 10 μM. 3. Intracellular recordings demonstrate a caffeine-evoked decrease in resting membrane potential, an increase in input resistance, a reduction of the long afterhyperpolarization (a.h.p.) and a decrease in accommodation. 4. The interaction between caffeine and adenosine was investigated on the extracellularly recorded e.p.s.p. The maximal response evoked by caffeine was increased in the presence of adenosine and the adenosine concentration-response curve was shifted to the right in a parallel fashion in the presence of caffeine. 5. It is suggested that the effects of the caffeine on hippocampal neurones may be mediated by a decrease of one or more potassium conductance(s), and that adenosine and caffeine may compete for the same electrophysiological active receptor site on these cells.",
author = "Greene, {R. W.} and Haas, {H. L.} and A. Hermann",
year = "1985",
language = "English (US)",
volume = "85",
pages = "163--169",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Effects of caffeine on hippocampal pyramidal cells in vitro

AU - Greene, R. W.

AU - Haas, H. L.

AU - Hermann, A.

PY - 1985

Y1 - 1985

N2 - 1. The effects of caffeine on the electrophysiological properties of CA1 pyramidal neurones were investigated in the rat hippocampal slice preparation in vitro. 2. A concentration-dependent increase in both the extracellularly recorded excitatory postsynaptic potential (e.p.s.p.) and the population spike resulting from stimulation of the stratum radiatum could be evoked by caffeine with a threshold concentration of 10 μM. 3. Intracellular recordings demonstrate a caffeine-evoked decrease in resting membrane potential, an increase in input resistance, a reduction of the long afterhyperpolarization (a.h.p.) and a decrease in accommodation. 4. The interaction between caffeine and adenosine was investigated on the extracellularly recorded e.p.s.p. The maximal response evoked by caffeine was increased in the presence of adenosine and the adenosine concentration-response curve was shifted to the right in a parallel fashion in the presence of caffeine. 5. It is suggested that the effects of the caffeine on hippocampal neurones may be mediated by a decrease of one or more potassium conductance(s), and that adenosine and caffeine may compete for the same electrophysiological active receptor site on these cells.

AB - 1. The effects of caffeine on the electrophysiological properties of CA1 pyramidal neurones were investigated in the rat hippocampal slice preparation in vitro. 2. A concentration-dependent increase in both the extracellularly recorded excitatory postsynaptic potential (e.p.s.p.) and the population spike resulting from stimulation of the stratum radiatum could be evoked by caffeine with a threshold concentration of 10 μM. 3. Intracellular recordings demonstrate a caffeine-evoked decrease in resting membrane potential, an increase in input resistance, a reduction of the long afterhyperpolarization (a.h.p.) and a decrease in accommodation. 4. The interaction between caffeine and adenosine was investigated on the extracellularly recorded e.p.s.p. The maximal response evoked by caffeine was increased in the presence of adenosine and the adenosine concentration-response curve was shifted to the right in a parallel fashion in the presence of caffeine. 5. It is suggested that the effects of the caffeine on hippocampal neurones may be mediated by a decrease of one or more potassium conductance(s), and that adenosine and caffeine may compete for the same electrophysiological active receptor site on these cells.

UR - http://www.scopus.com/inward/record.url?scp=0021963375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021963375&partnerID=8YFLogxK

M3 - Article

C2 - 4027463

AN - SCOPUS:0021963375

VL - 85

SP - 163

EP - 169

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 1

ER -