Effects of cyclooxygenase and lipoxygenase inhibition on eicosanoids and healing of acetic acid colitis in rats

Louise E. LeDuc, Kenny C. Su, Eve Guth, Terry Reedy, Paul H. Guth

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Both cyclooxygenase products, such as prostaglandin (PG) E2, and lipoxygenase products, such as leukotriene (LT) B4, are increased in colitis and have potent proinflammatory actions. We studied effects of specific inhibitors of cyclooxygenase and 5-lipoxygenase on the healing of acetic acid colitis in rats. Acetic acid colitis was induced 24 hr before enzyme inhibition began. Four days after induction of colitis, the area of gross colonic mucosal damage was determined by image analysis. Eicosanoid content in the intestinal lumen was quantitated by radioimmunoassay following chromatographic purification. Under these conditions, indomethacin significantly retarded the healing of colonic lesions and inhibited PGE2 by >90% compared to placebo-treated colitis rats. AA861 had no effect on the healing of lesions, although >75% inhibition of leukotriene synthesis was demonstrated. These results suggest that inhibition of endogenous colonic prostaglandins can impair healing mechanisms in acute colitis even after inflammation has developed. In contrast, inhibition of leukotriene synthesis did not affect healing.

Original languageEnglish (US)
Pages (from-to)289-294
Number of pages6
JournalDigestive Diseases and Sciences
Volume38
Issue number2
DOIs
StatePublished - Feb 1993

Fingerprint

Lipoxygenase
Eicosanoids
Colitis
Prostaglandin-Endoperoxide Synthases
Acetic Acid
Leukotrienes
Dinoprostone
Lipoxygenase Inhibitors
Gastrointestinal Contents
Leukotriene B4
Indomethacin
Prostaglandins
Radioimmunoassay
Placebos
Inflammation
Enzymes

Keywords

  • AA861
  • eicosanoids
  • indomethacin
  • inflammatory bowel disease
  • leukotrienes

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effects of cyclooxygenase and lipoxygenase inhibition on eicosanoids and healing of acetic acid colitis in rats. / LeDuc, Louise E.; Su, Kenny C.; Guth, Eve; Reedy, Terry; Guth, Paul H.

In: Digestive Diseases and Sciences, Vol. 38, No. 2, 02.1993, p. 289-294.

Research output: Contribution to journalArticle

LeDuc, Louise E. ; Su, Kenny C. ; Guth, Eve ; Reedy, Terry ; Guth, Paul H. / Effects of cyclooxygenase and lipoxygenase inhibition on eicosanoids and healing of acetic acid colitis in rats. In: Digestive Diseases and Sciences. 1993 ; Vol. 38, No. 2. pp. 289-294.
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