Effects of epidermal growth factor and insulin-like growth factor I on the levels of mRNA encoding aromatase cytochrome P-450 of human ovarian granulosa cells

Michael P. Steinkampf, Carole R. Mendelson, Evan R. Simpson

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

The effects of growth factors to regulate the activity of aromatase, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. Insulin-like growth factor I (IGF-I) increased aromatase activity as well as the synthesis of P-450AROM, in a concentration-dependent fashion. The levels of hybridizable mRNA species encoding cytochrome P-450AROM were also increased with IGF-I treatment. By contrast, epidermal growth factor (EGF) had no effect on these parameters when added alone, but markedly inhibited the action of follicle-stimulating hormone (FSH) to stimulate aromatase activity, and the synthesis of cytochrome P-450AROM as well as lts ability to increase the levels of mRNA encoding the enzyme. It is concluded that these growth factors have opposite effects on aromatase activity, and that these actions reflect, in part, changes in the synthesis of cytochrome P-450AROM which in turn are the consequence of changes in the levels of mRNA encoding this enzyme.

Original languageEnglish (US)
Pages (from-to)93-99
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume59
Issue number1-2
DOIs
StatePublished - Sep 1988

Keywords

  • Aromatase cytochrome P-450
  • Epidermal growth factor
  • Human
  • Insulin-like growth factor I
  • Ovarian granulosa cell

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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