TY - JOUR
T1 - Effects of epidermal growth factor and insulin-like growth factor I on the levels of mRNA encoding aromatase cytochrome P-450 of human ovarian granulosa cells
AU - Steinkampf, Michael P.
AU - Mendelson, Carole R.
AU - Simpson, Evan R.
N1 - Funding Information:
Address for correspondence: Evan R. Simpsor, The Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75235-9051, U.S.A. * Supported, in part, by USPHS Grants Nos. AM31206 and AGOO306. * * Supported, in part, by USPHS Training Gram No. 5-T32-HDO7190. Current address: Department of Obstetrics and Gynecology, University of Alabama at Birmingham, 619 South 19th Street, Birmingham, AL 35233, U.S.A.
PY - 1988/9
Y1 - 1988/9
N2 - The effects of growth factors to regulate the activity of aromatase, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. Insulin-like growth factor I (IGF-I) increased aromatase activity as well as the synthesis of P-450AROM, in a concentration-dependent fashion. The levels of hybridizable mRNA species encoding cytochrome P-450AROM were also increased with IGF-I treatment. By contrast, epidermal growth factor (EGF) had no effect on these parameters when added alone, but markedly inhibited the action of follicle-stimulating hormone (FSH) to stimulate aromatase activity, and the synthesis of cytochrome P-450AROM as well as lts ability to increase the levels of mRNA encoding the enzyme. It is concluded that these growth factors have opposite effects on aromatase activity, and that these actions reflect, in part, changes in the synthesis of cytochrome P-450AROM which in turn are the consequence of changes in the levels of mRNA encoding this enzyme.
AB - The effects of growth factors to regulate the activity of aromatase, as well as the synthesis of aromatase cytochrome P-450 (P-450AROM) have been studied in human ovarian granulosa cells obtained from women undergoing oocyte retrieval. Insulin-like growth factor I (IGF-I) increased aromatase activity as well as the synthesis of P-450AROM, in a concentration-dependent fashion. The levels of hybridizable mRNA species encoding cytochrome P-450AROM were also increased with IGF-I treatment. By contrast, epidermal growth factor (EGF) had no effect on these parameters when added alone, but markedly inhibited the action of follicle-stimulating hormone (FSH) to stimulate aromatase activity, and the synthesis of cytochrome P-450AROM as well as lts ability to increase the levels of mRNA encoding the enzyme. It is concluded that these growth factors have opposite effects on aromatase activity, and that these actions reflect, in part, changes in the synthesis of cytochrome P-450AROM which in turn are the consequence of changes in the levels of mRNA encoding this enzyme.
KW - Aromatase cytochrome P-450
KW - Epidermal growth factor
KW - Human
KW - Insulin-like growth factor I
KW - Ovarian granulosa cell
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U2 - 10.1016/0303-7207(88)90199-2
DO - 10.1016/0303-7207(88)90199-2
M3 - Article
C2 - 3264256
AN - SCOPUS:0023810901
SN - 0303-7207
VL - 59
SP - 93
EP - 99
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -