Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues

Ka Yaw Teo, Tenok O. DeHoyos, J. Craig Dutton, Frederick Grinnell, Bumsoo Han

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The two most significant challenges for successful cryopreservation of engineered tissues (ETs) are preserving tissue functionality and controlling highly tissue-type dependent preservation outcomes. In order to address these challenges, freezing-induced cell-fluid-matrix interactions should be understood, which determine the post-thaw cell viability and extracellular matrix (ECM) microstructure. However, the current understanding of this tissue-level biophysical interaction is still limited. In this study, freezing-induced cell-fluid-matrix interactions and their impact on the cells and ECM microstructure of ETs were investigated using dermal equivalents as a model ET. The dermal equivalents were constructed by seeding human dermal fibroblasts in type I collagen matrices with varying cell seeding density and collagen concentration. While these dermal equivalents underwent an identical freeze/thaw condition, their spatiotemporal deformation during freezing, post-thaw ECM microstructure, and cellular level cryoresponse were characterized. The results showed that the extent and characteristics of freezing-induced deformation were significantly different among the experimental groups, and the ETs with denser ECM microstructure experienced a larger deformation. The magnitude of the deformation was well correlated to the post-thaw ECM structure, suggesting that the freezing-induced deformation is a good indicator of post-thaw ECM structure. A significant difference in the extent of cellular injury was also noted among the experimental groups, and it depended on the extent of freezing-induced deformation of the ETs and the initial cytoskeleton organization. These results suggest that the cells have been subjected to mechanical insult due to the freezing-induced deformation as well as thermal insult. These findings provide insight on tissue-type dependent cryopreservation outcomes, and can help to design and modify cryopreservation protocols for new types of tissues from a pre-developed cryopreservation protocol.

Original languageEnglish (US)
Pages (from-to)5380-5390
Number of pages11
JournalBiomaterials
Volume32
Issue number23
DOIs
StatePublished - Aug 2011

Fingerprint

Freezing
Cell Communication
Extracellular Matrix
Tissue
Fluids
Cryopreservation
Skin
Microstructure
Collagen
Tissue Preservation
Collagen Type I
Cytoskeleton
Fibroblasts
Cell Survival
Cell Count
Hot Temperature
Cells
Wounds and Injuries

Keywords

  • Cell morphology
  • Cell viability
  • Cryopreservation
  • Dermal equivalent
  • ECM
  • Fibroblast

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. / Teo, Ka Yaw; DeHoyos, Tenok O.; Dutton, J. Craig; Grinnell, Frederick; Han, Bumsoo.

In: Biomaterials, Vol. 32, No. 23, 08.2011, p. 5380-5390.

Research output: Contribution to journalArticle

Teo, Ka Yaw ; DeHoyos, Tenok O. ; Dutton, J. Craig ; Grinnell, Frederick ; Han, Bumsoo. / Effects of freezing-induced cell-fluid-matrix interactions on the cells and extracellular matrix of engineered tissues. In: Biomaterials. 2011 ; Vol. 32, No. 23. pp. 5380-5390.
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