Effects of mitomycin on human colon carcinoma cells

B. H. Long, J. K V Willson, D. E. Brattain

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Subpopulations of malignant cells from primary cultures of human colon carcinoma were characterized with respect to their response to mitomycin (MMC). Growth inhibition assays indicated values of 2.06, 0.93, and 0.33 μM for the concentration of drug giving 50% inhibition of growth for sublines HCT 116b, HCT 116, and HCT 116a, respectively. Alkaline elution of filter-bound DNA from cells exposed to MMC in vitro showed a positive correlation between the amount of DNA cross-linking and growth inhibition as a function of drug concentration. Comparable DNA cross-linking was obtained at MMC concentrations of 10 μM for HCT 116b and 5 μM for HCT 116. The cross-linking of DNA from HCT 116a cells at 5 μM MMC was approximately equal to that from HCT 116 cells at doses between 10 and 20 μM MMC. Cross-link removal as a function of time after drug removal of MMC-treated cells was also measured. There was little difference in the rates of alkaline DNA elution after drug removal between HCT 116b and HCT 116a, suggesting that the ability to repair cross-links was not responsible for the differential sensitivities of the cells to MMC. The relative sensitivities of the subpopulations to MMC were reflected in vivo by MMC treatment of nude BALB/c mice bearing xenografts of the cultured sublines.

Original languageEnglish (US)
Pages (from-to)787-792
Number of pages6
JournalJournal of the National Cancer Institute
Volume73
Issue number4
Publication statusPublished - 1984

    Fingerprint

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Long, B. H., Willson, J. K. V., & Brattain, D. E. (1984). Effects of mitomycin on human colon carcinoma cells. Journal of the National Cancer Institute, 73(4), 787-792.