Effects of newly arachidonic acid metabolites on microsomal Ca++ binding, uptake and release

P. Kutsky, J R Falck, G. B. Weiss, S. Manna, N. Chacos, J. Capdevila

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The 5,6- 8,9-; 11,12- and 14,15-epoxyeicosatrienoic acids and their respective hydration products, the vic-doisl, recently reported as metabolites of arachidonic acid in rat liver microsomes, were examined for effect on release of 45Ca from canine aortic smooth muscle miscrosomes. At 10-6 M, the diols had no effect, but the 5,6-; 11,12- and 14,15-epoxyacids increased the loss of 45Ca. Further studies with the 14,15-epoxyacid demonstrated a dose-dependent decrease of Ca++ uptake (ATP present) in canine aortic microsomes in 0.03 mM Ca++, whereass Ca++ binding (ATP absent) was not affected. Ca++ uptake, binding and release in rat liver microsomes was similarly affected by the 14,15-epoxyacid, the major epoxyeicosatrienoic acid derivative produced by rat liver miscrosomal incubations. It is suggested that a alterations in Ca++ metabolism might be a possible mechanism of actions for these derivatives of arachidonic acid.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalProstaglandins
Volume26
Issue number1
DOIs
StatePublished - Jul 1983

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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