Effects of Single-Dose and Fractionated Cranial Irradiation on Rat Brain Accumulation of Methotrexate

Barton A. Kamen, John S. Holcenberg, Barbara J. Ring, Susan M. Adams, Larry E. Kun, Brian L. Fish, John E. Moulder

Research output: Contribution to journalArticle

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Abstract

The effects of single-dose and fractionated whole-brain irradiation on brain methotrexate (MTX) has been studied in a rat model. The amount of MTX present in the brain 24 hr after a single i.p. dose (100 mg/kg) was the same whether animals were sham irradiated or given a single dose of 2000 rads 6 or 48 hr prior to the drug (6.9, 8.3, and 6.8 pmol MTX/g, wet weight, respectively). Animals sham irradiated or given 2000 rads in 10 fractions over 11 days and treated with an average dose of 1.2 mg MTX/kg i.p. twice a week for 24 weeks did not differ significantly in their brain MTX concentration (7.9 and 8.3 pmol MTX/g, wet weight, respectively). Chronically MTX-treated animals became folate deficient whether they were irradiated or not (450 and 670 pmol folate/g, wet weight, brain in MTX-treated and control animals). Thus, MTX accumulates in the brain with acute or chronic administration, and this accumulation is not altered by this amount of brain irradiation.

Original languageEnglish (US)
Pages (from-to)5092-5094
Number of pages3
JournalCancer research
Volume44
Issue number11
StatePublished - Nov 1 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Kamen, B. A., Holcenberg, J. S., Ring, B. J., Adams, S. M., Kun, L. E., Fish, B. L., & Moulder, J. E. (1984). Effects of Single-Dose and Fractionated Cranial Irradiation on Rat Brain Accumulation of Methotrexate. Cancer research, 44(11), 5092-5094.