Efficient inducible Cre-mediated recombination in Tcf21cell lineages in the heart and kidney

Asha Acharya, Seung Tae Baek, Serena Banfi, Banu Eskiocak, Michelle D. Tallquist

Research output: Contribution to journalArticle

76 Scopus citations

Abstract

Tcf21 is a Class II bHLH family member with essential roles in the formation of the lungs, kidneys, gonads, spleen, and heart. Here, we report the utility of a mouse line with targeted insertion of a tamoxifen-inducible Cre recombinase, MerCreMer at the Tcf21 locus. This mouse line will permit the inducible expression of Cre recombinase in Tcf21-expressing cells. Using ROSA26 reporter mice, we show that Cre recombinase is specifically and robustly activated in multiple Tcf21-expressing tissues during embryonic and postnatal development. The expression profile in the kidney is particularly dynamic with the ability to cause recombination in mesangial cells at one time of induction and podocytes at another time. These features make the Tcf21-driven inducible Cre line (Tcf21 iCre) a valuable genetic tool for spatiotemporal gene function analysis and lineage tracing of cells in the heart, kidney, cranial muscle, and gonads.

Original languageEnglish (US)
Pages (from-to)870-877
Number of pages8
JournalGenesis
Volume49
Issue number11
DOIs
StatePublished - Nov 1 2011

Keywords

  • Heart
  • Inducible-Cre
  • Kidney
  • Lineage tracing
  • Tcf21

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

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    Acharya, A., Baek, S. T., Banfi, S., Eskiocak, B., & Tallquist, M. D. (2011). Efficient inducible Cre-mediated recombination in Tcf21cell lineages in the heart and kidney. Genesis, 49(11), 870-877. https://doi.org/10.1002/dvg.20750