TY - JOUR
T1 - EGFR signaling regulates the proliferation of Drosophila adult midgut progenitors
AU - Jiang, Huaqi
AU - Edgar, Bruce A.
PY - 2009/2/1
Y1 - 2009/2/1
N2 - In holometabolous insects, the adult appendages and internal organs form anew from larval progenitor cells during metamorphosis. As described here, the adult Drosophila midgut, including intestinal stem cells (ISCs), develops from adult midgut progenitor cells (AMPs) that proliferate during larval development in two phases. Dividing AMPs first disperse, but later proliferate within distinct islands, forming large cell clusters that eventually fuse during metamorphosis to make the adult midgut epithelium. We find that signaling through the EGFR/RAS/ MAPK pathway is necessary and limiting for AMP proliferation. Midgut visceral muscle produces a weak EGFR ligand, Vein, which is required for early AMP proliferation. Two stronger EGFR ligands, Spitz and Keren, are expressed by the AMPs themselves and provide an additional, autocrine mitogenic stimulus to the AMPs during late larval stages.
AB - In holometabolous insects, the adult appendages and internal organs form anew from larval progenitor cells during metamorphosis. As described here, the adult Drosophila midgut, including intestinal stem cells (ISCs), develops from adult midgut progenitor cells (AMPs) that proliferate during larval development in two phases. Dividing AMPs first disperse, but later proliferate within distinct islands, forming large cell clusters that eventually fuse during metamorphosis to make the adult midgut epithelium. We find that signaling through the EGFR/RAS/ MAPK pathway is necessary and limiting for AMP proliferation. Midgut visceral muscle produces a weak EGFR ligand, Vein, which is required for early AMP proliferation. Two stronger EGFR ligands, Spitz and Keren, are expressed by the AMPs themselves and provide an additional, autocrine mitogenic stimulus to the AMPs during late larval stages.
KW - Adult midgut progenitor (AMP)
KW - EGFR
KW - Intestinal stem cell (ISC)
UR - http://www.scopus.com/inward/record.url?scp=64549110803&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64549110803&partnerID=8YFLogxK
U2 - 10.1242/dev.026955
DO - 10.1242/dev.026955
M3 - Article
C2 - 19141677
AN - SCOPUS:64549110803
VL - 136
SP - 483
EP - 493
JO - Journal of Embryology and Experimental Morphology
JF - Journal of Embryology and Experimental Morphology
SN - 0950-1991
IS - 3
ER -