Elevated expression of phosphatidylserine in the outer membrane leaflet of human tumor cells and recognition by activated human blood monocytes

Teruhiro Utsugi, Alan J. Schroit, Jerome Connor, Corazon D. Bucana, Isaiah J. Fidler

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Abstract

We determined whether the presence of phosphatidylserine (PS) in the outer membrane leaflet of human tumor cells correlated with their recognition by activated human monocytes. Three tumorigenic cell lines, A375 melanoma and A431 and Colo-16 carcinomas, and a normal human epidermal keratinocyte line (NHEK) were incubated with monocytes activated to the tumoricidal state by γ-interferon and lipopolysaccharide. Activated human monocytes bound to and lysed all tumorigenic targets, while the nontumorigenic NHEK were neither bound nor killed. Semi-quantitative analysis of PS in the outer leaflet of the cells revealed that the tumorigenic cells expressed 3-7-fold more PS than did the nontumorigenic NHEK. To determine whether enhanced PS expression on the tumor cells was responsible for their recognition by activated monocytes, NHEK were supplemented with exogenously supplied analogues of PS and phosphatidylcholine. PS-labeled NHEK but not phosphatidylcholine-labeled nor control NHEK bound to activated human monocytes. These results suggest a role for PS in monocyte recognition of tumor cells.

Original languageEnglish (US)
Pages (from-to)3062-3066
Number of pages5
JournalCancer Research
Volume51
Issue number11
StatePublished - Jun 1 1991

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Phosphatidylserines
Monocytes
Keratinocytes
Membranes
Neoplasms
Phosphatidylcholines
Interferons
Lipopolysaccharides
Melanoma
Carcinoma
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Elevated expression of phosphatidylserine in the outer membrane leaflet of human tumor cells and recognition by activated human blood monocytes. / Utsugi, Teruhiro; Schroit, Alan J.; Connor, Jerome; Bucana, Corazon D.; Fidler, Isaiah J.

In: Cancer Research, Vol. 51, No. 11, 01.06.1991, p. 3062-3066.

Research output: Contribution to journalArticle

Utsugi, Teruhiro ; Schroit, Alan J. ; Connor, Jerome ; Bucana, Corazon D. ; Fidler, Isaiah J. / Elevated expression of phosphatidylserine in the outer membrane leaflet of human tumor cells and recognition by activated human blood monocytes. In: Cancer Research. 1991 ; Vol. 51, No. 11. pp. 3062-3066.
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