Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury

Jianlin Chen, Martin M. Matzuk, Xin J. Zhou, Christopher Y. Lu

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Toll-like receptor 4 (TLR4), a receptor for damage-associated molecular pattern molecules and also the lipopolysaccharide receptor, is required for early endothelial activation leading to maximal inflammation and injury during murine ischemic acute kidney injury. DNA microarray analysis of ischemic kidneys from TLR4-sufficient and-deficient mice showed that pentraxin 3 (PTX3) was upregulated only on the former while transgenic knockout of PTX3 ameliorated acute kidney injury. PTX3 was expressed predominantly on peritubular endothelia of the outer medulla of the kidney in control mice. Acute kidney injury increased PTX3 protein in the kidney and the plasma where it may be a biomarker of the injury. Stimulation by hydrogen peroxide, or the TLR4 ligands recombinant human high-mobility group protein B1 or lipopolysaccharide, induced PTX3 expression in the Mile Sven 1 endothelial cell line and in primary renal endothelial cells, suggesting that endothelial PTX3 was induced by pathways involving TLR4 and reactive oxygen species. This increase was inhibited by conditional endothelial knockout of myeloid differentiation primary response gene 88, a mediator of a TLR4 intracellular signaling pathway. Compared to wild-type mice, PTX3 knockout mice had decreased endothelial expression of cell adhesion molecules at 4 h of reperfusion, possibly contributing to a decreased early maladaptive inflammation in the kidneys of knockout mice. At 24 h of reperfusion, PTX3 knockout increased expression of endothelial adhesion molecules when regulatory and reparative leukocytes enter the kidney. Thus, endothelial PTX3 plays a pivotal role in the pathogenesis of ischemic acute kidney injury.

Original languageEnglish (US)
Pages (from-to)1195-1207
Number of pages13
JournalKidney International
Volume82
Issue number11
DOIs
StatePublished - Dec 1 2012

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Acute Kidney Injury
Toll-Like Receptor 4
Kidney
Endothelial Cells
Knockout Mice
Reperfusion
CD14 Antigens
Kidney Medulla
High Mobility Group Proteins
Inflammation
PTX3 protein
Wounds and Injuries
Cell Adhesion Molecules
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Hydrogen Peroxide
Endothelium
Lipopolysaccharides
Reactive Oxygen Species
Leukocytes

Keywords

  • acute kidney injury
  • acute renal failure
  • endothelial cells
  • renal ischemia-reperfusion injury

ASJC Scopus subject areas

  • Nephrology

Cite this

Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury. / Chen, Jianlin; Matzuk, Martin M.; Zhou, Xin J.; Lu, Christopher Y.

In: Kidney International, Vol. 82, No. 11, 01.12.2012, p. 1195-1207.

Research output: Contribution to journalArticle

Chen, Jianlin ; Matzuk, Martin M. ; Zhou, Xin J. ; Lu, Christopher Y. / Endothelial pentraxin 3 contributes to murine ischemic acute kidney injury. In: Kidney International. 2012 ; Vol. 82, No. 11. pp. 1195-1207.
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