Endothelin-1 immunoreactivity and mRNA in the transplanted human heart

A. Giaid, D. Saleh, Masashi Yanagisawa, R. D C Forbes

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Endothelin-1 (ET-1) is a 21-residue peptide produced by endothelial cells and possesses a wide range of biological activities, including vasoconstriction, mitogenesis, and inotropic effects on the heart. The aim of the present study was to determine the cellular localization of ET-1 immunoreactivity and mRNA in routine endomyocardial biopsy specimens of transplanted human hearts, and to correlate the findings with the associated histological changes. Multiple-step paraffin sections of 72 biopsy samples were immunostained with antiserum to ET-1 and von Willebrand factor (factor VIII) using the avidin-biotin-peroxidase complex method. ET-1 immunoreactivity was localized to vascular and endocardial endothelial cells, as well as to cardiomyocytes. The pattern of endothelial cell immunostaining with the ET-1 antiserum was similar to that of factor VIII. Previous biopsy sites and areas of granulation tissue appeared to have greater ET-1 immunoreactivity, particularly in sections immunostained with the ET-1 antiserum. There was a significant correlation between the presence of ET-1 immunoreactivity and fibrosis or granulation tissue in the biopsy specimens (P<0.03). There was no correlation between ET-1 immunoreactivity and the presence of cellular infiltrate, definitive rejection, or Quilty effect. In situ hybridization with radiolabeled RNA probes revealed expression of ET-1 mRNA in endothelial cells and myocytes, also in association with granulation tissue and fibrosis. No cellular reactivity was present in control sections stained with the ET-1 antiserum preadsorped with its synthetic peptide. The findings suggests a possible role for ET-1 in vascular regeneration and angiogenesis following myocardial injury.

Original languageEnglish (US)
Pages (from-to)1308-1313
Number of pages6
JournalTransplantation
Volume59
Issue number9
StatePublished - 1995

Fingerprint

Endothelin-1
Messenger RNA
Granulation Tissue
Immune Sera
Endothelial Cells
Biopsy
Fibrosis
RNA Probes
Peptides
Avidin
Factor VIII
Biotin
Vasoconstriction
Cardiac Myocytes
Paraffin
Muscle Cells
Peroxidase
In Situ Hybridization
Blood Vessels
Regeneration

ASJC Scopus subject areas

  • Immunology
  • Transplantation

Cite this

Giaid, A., Saleh, D., Yanagisawa, M., & Forbes, R. D. C. (1995). Endothelin-1 immunoreactivity and mRNA in the transplanted human heart. Transplantation, 59(9), 1308-1313.

Endothelin-1 immunoreactivity and mRNA in the transplanted human heart. / Giaid, A.; Saleh, D.; Yanagisawa, Masashi; Forbes, R. D C.

In: Transplantation, Vol. 59, No. 9, 1995, p. 1308-1313.

Research output: Contribution to journalArticle

Giaid, A, Saleh, D, Yanagisawa, M & Forbes, RDC 1995, 'Endothelin-1 immunoreactivity and mRNA in the transplanted human heart', Transplantation, vol. 59, no. 9, pp. 1308-1313.
Giaid, A. ; Saleh, D. ; Yanagisawa, Masashi ; Forbes, R. D C. / Endothelin-1 immunoreactivity and mRNA in the transplanted human heart. In: Transplantation. 1995 ; Vol. 59, No. 9. pp. 1308-1313.
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