Endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in vascular smooth muscle

K. Goto, Y. Kasuya, N. Matsuki, Y. Takuwa, H. Kurihara, T. Ishikawa, S. Kimura, Masashi Yanagisawa, T. Masaki

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

Endothelin is a potent endothelium-derived vasoconstrictor peptide recently characterized from porcine and human vascular endothelial cells. Here we provide evidence that endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in porcine coronary artery smooth muscle. The vasoconstrictor action of endothelin is efficiently antagonized by low doses of the dihydropyridine Ca2+-channel blocker nicardipine. Endothelin augments the Ca2+-induced contraction in a high-K+ depolarizing solution, markedly enhances high-threshold Ca2+-channel current on the whole-cell patch clamp recording, and causes a sustained increase in the intracellular Ca2+ that is largely dependent on extracellular Ca2+. These findings suggest that endothelin exerts its vasoconstrictor effect by either directly or indirectly activating the voltage-dependent Ca2+ channel.

Original languageEnglish (US)
Pages (from-to)3915-3918
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number10
StatePublished - 1989

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Endothelins
Vascular Smooth Muscle
Vasoconstrictor Agents
Swine
Nicardipine
Endothelium
Smooth Muscle
Coronary Vessels
Endothelial Cells
1,4-dihydropyridine
Peptides

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in vascular smooth muscle. / Goto, K.; Kasuya, Y.; Matsuki, N.; Takuwa, Y.; Kurihara, H.; Ishikawa, T.; Kimura, S.; Yanagisawa, Masashi; Masaki, T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, No. 10, 1989, p. 3915-3918.

Research output: Contribution to journalArticle

Goto, K. ; Kasuya, Y. ; Matsuki, N. ; Takuwa, Y. ; Kurihara, H. ; Ishikawa, T. ; Kimura, S. ; Yanagisawa, Masashi ; Masaki, T. / Endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in vascular smooth muscle. In: Proceedings of the National Academy of Sciences of the United States of America. 1989 ; Vol. 86, No. 10. pp. 3915-3918.
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AU - Kasuya, Y.

AU - Matsuki, N.

AU - Takuwa, Y.

AU - Kurihara, H.

AU - Ishikawa, T.

AU - Kimura, S.

AU - Yanagisawa, Masashi

AU - Masaki, T.

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AB - Endothelin is a potent endothelium-derived vasoconstrictor peptide recently characterized from porcine and human vascular endothelial cells. Here we provide evidence that endothelin activates the dihydropyridine-sensitive, voltage-dependent Ca2+ channel in porcine coronary artery smooth muscle. The vasoconstrictor action of endothelin is efficiently antagonized by low doses of the dihydropyridine Ca2+-channel blocker nicardipine. Endothelin augments the Ca2+-induced contraction in a high-K+ depolarizing solution, markedly enhances high-threshold Ca2+-channel current on the whole-cell patch clamp recording, and causes a sustained increase in the intracellular Ca2+ that is largely dependent on extracellular Ca2+. These findings suggest that endothelin exerts its vasoconstrictor effect by either directly or indirectly activating the voltage-dependent Ca2+ channel.

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