Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease

D. Rachmilewitz, J. S. Stamler, D. Bachwich, F. Karmeli, Z. Ackerman, D. K. Podolsky

Research output: Contribution to journalArticle

301 Citations (Scopus)

Abstract

Recent studies have suggested that nitric oxide (NO·), the product of nitric oxide synthase in inflammatory cells, may play a part in tissue injury and inflammation through its oxidative metabolism. In this study the colonic generation of oxides of nitrogen (NO(x)) and nitric oxide synthase determined in ulcerative Crohn's disease. Colonic biopsy specimens were obtained from inflammatory bowel disease patients and from normal controls. Mucosal explants were cultured in vitro for 24 hours and NO(x) generation was determined. Nitric oxide synthase activity was monitored by the conversion of [3H]-L-arginine to citrulline. Median NO(x) generation by inflamed colonic mucosa of patients with active ulcerative colitis and Crohn's colitis was 4.2- and 8.1-fold respectively higher than that by normal human colonic mucosa. In ulcerative colitis and Crohn's colitis nitric oxide synthase activity was 10.0- and 3.8-fold respectively higher than in normal subjects. Colonic NO(x) generation is significantly decreased by methylprednisolone and ketotifen. The decrease in NO(x) generation by cultured colonic mucosa induced by methylprednisolone suggests that NO synthase activity is induced during the culture and the steroid effect may contribute to its therapeutic effect. Enhanced colonic NO(x) generation by stimulated nitric oxide synthase activity in ulcerative colitis and Crohn's disease may contribute to tissue injury.

Original languageEnglish (US)
Pages (from-to)718-723
Number of pages6
JournalGut
Volume36
Issue number5
StatePublished - 1995

Fingerprint

Ulcerative Colitis
Nitric Oxide Synthase
Crohn Disease
Nitric Oxide
Mucous Membrane
Methylprednisolone
Colitis
Ketotifen
Citrulline
Wounds and Injuries
Therapeutic Uses
Inflammatory Bowel Diseases
Oxides
Arginine
Steroids
Inflammation
Biopsy

Keywords

  • Crohn's disease
  • Nitric oxide
  • Ulcerative colitis

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Rachmilewitz, D., Stamler, J. S., Bachwich, D., Karmeli, F., Ackerman, Z., & Podolsky, D. K. (1995). Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease. Gut, 36(5), 718-723.

Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease. / Rachmilewitz, D.; Stamler, J. S.; Bachwich, D.; Karmeli, F.; Ackerman, Z.; Podolsky, D. K.

In: Gut, Vol. 36, No. 5, 1995, p. 718-723.

Research output: Contribution to journalArticle

Rachmilewitz, D, Stamler, JS, Bachwich, D, Karmeli, F, Ackerman, Z & Podolsky, DK 1995, 'Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease', Gut, vol. 36, no. 5, pp. 718-723.
Rachmilewitz, D. ; Stamler, J. S. ; Bachwich, D. ; Karmeli, F. ; Ackerman, Z. ; Podolsky, D. K. / Enhanced colonic nitric oxide generation and nitric oxide synthase activity in ulcerative colitis and Crohn's disease. In: Gut. 1995 ; Vol. 36, No. 5. pp. 718-723.
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