Enhanced neurochemical profile of the rat brain using in vivo (1)H NMR spectroscopy at 16.4 T.

Sung Tak Hong, Dávid Zsolt Balla, G. Shajan, Changho Choi, Kâmil Uǧurbil, Rolf Pohmann

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Abstract

Single voxel magnetic resonance spectroscopy with ultrashort echo time was implemented at 16.4 T to enhance the neurochemical profile of the rat brain in vivo. A TE of 1.7 msec was achieved by sequence optimization and by using short-duration asymmetric pulses. Macromolecular signal components were parameterized individually and included in the quantitative analysis, replacing the use of a metabolite-nulled spectrum. Because of the high spectral dispersion, several signals close to the water line could be detected, and adjacent peaks could be resolved. All 20 metabolites detected in this study were quantified with Cramér-Rao lower bounds below 20%, implying reliable quantification accuracy. The signal of acetate was detected for the first time in rat brain in vivo with Cramér-Rao lower bounds of 16% and a concentration of 0.52 μmol/g. The absolute concentrations of most metabolites showed close agreement with values previously measured using in vivo (1)H NMR spectroscopy and in vitro biochemical assay.

Original languageEnglish (US)
Pages (from-to)28-34
Number of pages7
JournalMagnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine
Volume65
Issue number1
StatePublished - Jan 2011

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ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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