2-Methoxyestradiol (2-ME) is an endogenous estradiol metabolite that disrupts microtubule function, suppresses murine tumors, and inhibits angiogenesis. Since some microtubule inhibitors have been shown to alter radiosensitivity, we have evaluated 2-ME as a radiation enhancer in vitro. H460 human lung cancer cells were plated, treated with 2-ME for 24 h, and irradiated; then colony-forming ability was assessed. The radiation dose enhancement ratios (DERs) using this protocol were 1.3, 1.8 and 2.1 for 1, 1.5 and 2 μM 2-ME, respectively. Using a single-cell plating protocol, the respective DERs were 1.2, 1.5 and 1.8. The parent compound of 2-ME, β- estradiol, did not enhance radiation effects at equally cytotoxic doses. Isobologram analysis showed that 1 μM 2-ME was additive with radiation, but that 1.5 and 2 μM were synergistic. Cell cycle analysis showed a dose- dependent increase in the percentage of cells in the radiosensitive G2/M phase after a 24-h treatment with 2-ME; a threefold increase in the percentage of cells in G2/M phase was observed using 2 μM 2-ME. Treatment with 2 μM 2-ME almost completely inhibited repair of sublethal damage (SLD) as shown using split-dose recovery. Radiosensitive, repair-deficient murine SCID (severe combined immunodeficient) cells did not show enhancement of radiation effects with 2 μM 2-ME, but enhancement was observed in the wild- type parental cells (CB-17). SCID cells complemented with human DNA-dependent protein kinase restored radioenhancement by 2-ME. In addition, MCF-7 breast cancer cells were also radiosensitized by 2 μM 2-ME (DER = 2.1). These data suggest that 2-ME is a potential radiation sensitizer, in addition to its previously reported antitumor and antiangiogenic properties. We have verified the antiangiogenic activity of 2-ME in vitro using human endothelial cells. Based on these results, we hypothesize that the mechanism of radiation enhancement may involve redistribution of cells into G2/M phase by 2-ME, and that the resulting population of cells is repair-deficient and thus radiosensitive. (C) 2000 by Radiation Research Society.
|Original language||English (US)|
|Number of pages||8|
|Publication status||Published - Apr 2000|
ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Radiology Nuclear Medicine and imaging