Enhancer RNA facilitates NELF release from immediate early genes

Katie Schaukowitch, Jae Yeol Joo, Xihui Liu, Jonathan K. Watts, Carlos Martinez, Tae Kyung Kim

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

Enhancer RNAs (eRNAs) are a class of long noncoding RNAs (lncRNA) expressed from active enhancers, whose function and action mechanism are yet to befirmly established. Here we show that eRNAs facilitate the transition of paused RNA polymerase II (RNAPII) into productive elongation by acting as a decoy for the negative elongation factor (NELF) complex upon induction of immediate early genes (IEGs) in neurons. eRNAs are synthesized priorto the culmination of target gene transcription andinteract with the NELF complex. Knockdown of eRNAs expressed at neuronal enhancers impairs transient release of NELF from the specific target promoters during transcriptional activation, coinciding with a decrease in target mRNA induction. The enhancer-promoter interaction was unaffected by eRNA knockdown. Instead, chromatin looping might enable eRNAs to act locally at a specific promoter. Our findings highlight the spatiotemporally regulated action mechanism of eRNAs during early transcriptional elongation.

Original languageEnglish (US)
Pages (from-to)29-42
Number of pages14
JournalMolecular Cell
Volume56
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Immediate-Early Genes
RNA
Long Noncoding RNA
RNA Polymerase II
negative elongation factor
Transcriptional Activation
Chromatin
Neurons
Messenger RNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Medicine(all)

Cite this

Schaukowitch, K., Joo, J. Y., Liu, X., Watts, J. K., Martinez, C., & Kim, T. K. (2014). Enhancer RNA facilitates NELF release from immediate early genes. Molecular Cell, 56(1), 29-42. https://doi.org/10.1016/j.molcel.2014.08.023

Enhancer RNA facilitates NELF release from immediate early genes. / Schaukowitch, Katie; Joo, Jae Yeol; Liu, Xihui; Watts, Jonathan K.; Martinez, Carlos; Kim, Tae Kyung.

In: Molecular Cell, Vol. 56, No. 1, 2014, p. 29-42.

Research output: Contribution to journalArticle

Schaukowitch, K, Joo, JY, Liu, X, Watts, JK, Martinez, C & Kim, TK 2014, 'Enhancer RNA facilitates NELF release from immediate early genes', Molecular Cell, vol. 56, no. 1, pp. 29-42. https://doi.org/10.1016/j.molcel.2014.08.023
Schaukowitch, Katie ; Joo, Jae Yeol ; Liu, Xihui ; Watts, Jonathan K. ; Martinez, Carlos ; Kim, Tae Kyung. / Enhancer RNA facilitates NELF release from immediate early genes. In: Molecular Cell. 2014 ; Vol. 56, No. 1. pp. 29-42.
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