TY - JOUR
T1 - Enzymatic probe sonication as a tool for solid-liquid extraction for total selenium determination by electrothermal-atomic absorption spectrometry
AU - Vale, G.
AU - Pereira, S.
AU - Mota, A.
AU - Fonseca, L.
AU - Capelo, J. L.
N1 - Funding Information:
We thank FCT (Science and Technical Foundation), from Portugal for Financial support under Project MetalControl N-1734. We would also like to thank the fruitful work done by the reviewers of this manuscript. The research findings reported in this work are protected by international laws under patent request PORT No. 23848 of the INI, Instituto Nacional da Propiedade Industrial, Portugal.
PY - 2007/11/30
Y1 - 2007/11/30
N2 - A new fast and reproducible approach is described for the application of the enzymatic probe sonication (EPS) methodology [J.L. Capelo, P. Ximénez-Embún, Y. Madrid-Albarrán, C. Cámara, Anal. Chem. 76 (2004) 233-237] for total selenium determination by electrothermal atomic absorption spectrometry, ET-AAS. Ni(NO3)2 and Pd(NO3)2 were studied as matrix modifiers in conjunction with H2O2, being best results obtained with Pd(NO3)2 plus H2O2. The presence of H2O2 as matrix modifier increases up to 66% the time-life of the graphite tubes, by avoiding the building-up of carbonaceous residues. BCR-414 plankton and ERM-CE 278 mussel tissue reference materials were used for proof-of-the-methodology. Different enzymes, protease XIV, substilisin and trypsin were studied. The use of fresh enzyme was found critical. Good Se recoveries were obtained for oyster tissue, 111%; BCR-414 plankton, 106% and ERM-CE 278 mussel tissue, 93%, when protease XIV was used. Data regarding microwave digestion versus EPS methodology is also presented and discussed.
AB - A new fast and reproducible approach is described for the application of the enzymatic probe sonication (EPS) methodology [J.L. Capelo, P. Ximénez-Embún, Y. Madrid-Albarrán, C. Cámara, Anal. Chem. 76 (2004) 233-237] for total selenium determination by electrothermal atomic absorption spectrometry, ET-AAS. Ni(NO3)2 and Pd(NO3)2 were studied as matrix modifiers in conjunction with H2O2, being best results obtained with Pd(NO3)2 plus H2O2. The presence of H2O2 as matrix modifier increases up to 66% the time-life of the graphite tubes, by avoiding the building-up of carbonaceous residues. BCR-414 plankton and ERM-CE 278 mussel tissue reference materials were used for proof-of-the-methodology. Different enzymes, protease XIV, substilisin and trypsin were studied. The use of fresh enzyme was found critical. Good Se recoveries were obtained for oyster tissue, 111%; BCR-414 plankton, 106% and ERM-CE 278 mussel tissue, 93%, when protease XIV was used. Data regarding microwave digestion versus EPS methodology is also presented and discussed.
KW - EPS
KW - ET-AAS
KW - Enzymes
KW - Focused ultrasound
KW - Selenium
KW - Solid-liquid extraction
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U2 - 10.1016/j.talanta.2007.05.051
DO - 10.1016/j.talanta.2007.05.051
M3 - Article
C2 - 18371630
AN - SCOPUS:36049006849
SN - 0039-9140
VL - 74
SP - 198
EP - 205
JO - Talanta
JF - Talanta
IS - 2
ER -