Epitope mapping of anti-rhodopsin antibodies from patients with normal pressure glaucoma

Carmelo Romano, Zhengzhi Li, Anatol Arendt, Paul A. Hargrave, Martin B. Wax

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

PURPOSE. The presence of anti-rhodopsin antibodies in patients with normal pressure glaucoma (NPG) has been previously demonstrated with western blot analysis and enzyme-linked immunosorbent assay. To learn more about the characteristics, origin, and possible significance of these antibodies, the epitopic specificity of the anti-rhodopsin antibodies was examined in four NPG patients. METHODS. Antibodies in patient sera were assayed by western blot analysis against purified bovine rhodopsin. Peptides derived from particular segments of the rhodopsin sequence were tested for activity in competing for rhodopsin-antibody binding. RESULTS. Of a series of nine peptides that constitute most of the hydrophilic regions of rhodopsin, only one, consisting of the C-terminal 25 amino acids, prevented binding of the patient antibodies to rhodopsin. Higher resolution mapping using a set of dodecamers of overlapping sequences from the C-terminal region demonstrated that antibody binding is completely dependent on the last two amino acids. Removing the C-terminal alanine alone, or amidating the C terminus carboxyl group, also eliminated antibody binding. CONCLUSIONS. Because four of four patient antibodies examined exhibited the identical epitopic specificity, it is likely that a common mechanism underlies their generation. This may indicate that molecular mimicry has occurred, because several pathogens contain similar C-terminal sequences. Although they may serve as diagnostic markers, and provide evidence that there is an autoimmune component in some patients with glaucoma, the role, if any, that these antibodies play in the pathogenesis of the disease remains unknown.

Original languageEnglish (US)
Pages (from-to)1275-1280
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number6
StatePublished - May 1999

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Epitope Mapping
Rhodopsin
Glaucoma
Anti-Idiotypic Antibodies
Pressure
Antibodies
Western Blotting
Molecular Mimicry
Amino Acids
Peptides
Antibody Specificity
Alanine
Enzyme-Linked Immunosorbent Assay
Serum

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Romano, C., Li, Z., Arendt, A., Hargrave, P. A., & Wax, M. B. (1999). Epitope mapping of anti-rhodopsin antibodies from patients with normal pressure glaucoma. Investigative Ophthalmology and Visual Science, 40(6), 1275-1280.

Epitope mapping of anti-rhodopsin antibodies from patients with normal pressure glaucoma. / Romano, Carmelo; Li, Zhengzhi; Arendt, Anatol; Hargrave, Paul A.; Wax, Martin B.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 6, 05.1999, p. 1275-1280.

Research output: Contribution to journalArticle

Romano, C, Li, Z, Arendt, A, Hargrave, PA & Wax, MB 1999, 'Epitope mapping of anti-rhodopsin antibodies from patients with normal pressure glaucoma', Investigative Ophthalmology and Visual Science, vol. 40, no. 6, pp. 1275-1280.
Romano, Carmelo ; Li, Zhengzhi ; Arendt, Anatol ; Hargrave, Paul A. ; Wax, Martin B. / Epitope mapping of anti-rhodopsin antibodies from patients with normal pressure glaucoma. In: Investigative Ophthalmology and Visual Science. 1999 ; Vol. 40, No. 6. pp. 1275-1280.
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N2 - PURPOSE. The presence of anti-rhodopsin antibodies in patients with normal pressure glaucoma (NPG) has been previously demonstrated with western blot analysis and enzyme-linked immunosorbent assay. To learn more about the characteristics, origin, and possible significance of these antibodies, the epitopic specificity of the anti-rhodopsin antibodies was examined in four NPG patients. METHODS. Antibodies in patient sera were assayed by western blot analysis against purified bovine rhodopsin. Peptides derived from particular segments of the rhodopsin sequence were tested for activity in competing for rhodopsin-antibody binding. RESULTS. Of a series of nine peptides that constitute most of the hydrophilic regions of rhodopsin, only one, consisting of the C-terminal 25 amino acids, prevented binding of the patient antibodies to rhodopsin. Higher resolution mapping using a set of dodecamers of overlapping sequences from the C-terminal region demonstrated that antibody binding is completely dependent on the last two amino acids. Removing the C-terminal alanine alone, or amidating the C terminus carboxyl group, also eliminated antibody binding. CONCLUSIONS. Because four of four patient antibodies examined exhibited the identical epitopic specificity, it is likely that a common mechanism underlies their generation. This may indicate that molecular mimicry has occurred, because several pathogens contain similar C-terminal sequences. Although they may serve as diagnostic markers, and provide evidence that there is an autoimmune component in some patients with glaucoma, the role, if any, that these antibodies play in the pathogenesis of the disease remains unknown.

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