Epoxyeicosatrienoic acids are released to mediate shear stress-dependent hyperpolarization of arteriolar smooth muscle

An Huang, Dong Sun, Azita Jacobson, Mairead A. Carroll, J R Falck, Gabor Kaley

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

We hypothesized that shear stress stimulates the release of epoxyeicosatrienoic acids (EETs) from arteriolar endothelium, which directly hyperpolarize smooth muscle. To test this hypothesis, a perfusion system, consisting of two separate, serially connected chambers (A and B), was used. A donor vessel, isolated from gracilis muscle of female NO-deficient mice and rats, was cannulated in chamber A. In chamber B, an endothelium-denuded detector vessel isolated from mesentery of these animals was cannulated. In the presence of indomethacin, 5, 10, and 20 dyne/cm2 shear stress elicited dilation of donor vessels, followed by dilation of detector vessels. Changes in membrane potential of the detector vessel smooth muscle cells in response to the perfusate from 5 and 10 dyne/cm2 shear stress-stimulated donor vessels was also recorded (by ≈=-12 to -15 and -20 to -30 mV, respectively). Exposing detector vessels to 30 mmol/L KCl or pretreating them with iberiotoxin abolished their hyperpolarization and dilation to the flow of perfusate. Pretreatment of donor vessels with PPOH, an inhibitor of cytochrome P-450/epoxygenase, eliminated dilator responses in both donor and detector vessels, as well as the hyperpolarization of detector vessels. GC-MS analysis showed increasing release of EETs into the perfusate collected from 1, 5, and 10 dyne/cm2 shear stress-stimulated arterioles, which was abolished by PPOH. Thus, EETs, released from endothelial cells of donor vessels stimulated with shear stress, hyperpolarize smooth muscle of downstream detector vessels, confirming their identity as endothelium-derived hyperpolarizing factors and suggesting that gap junctional communication may not be necessary for shear stress-stimulated EDHF-mediated vasodilation.

Original languageEnglish (US)
Pages (from-to)376-383
Number of pages8
JournalCirculation Research
Volume96
Issue number3
DOIs
StatePublished - Feb 18 2005

Fingerprint

Endothelium
Smooth Muscle
Dilatation
Acids
Mesentery
Arterioles
Vasodilation
Indomethacin
Membrane Potentials
Smooth Muscle Myocytes
Endothelial Cells
Perfusion
Communication
propenylphosphonic acid
Gracilis Muscle
iberiotoxin
Cytochrome P-450 Enzyme Inhibitors
endothelium-dependent hyperpolarization factor

Keywords

  • Arterioles
  • EET
  • Hyperpolarization
  • NO deficiency
  • Shear stress

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Epoxyeicosatrienoic acids are released to mediate shear stress-dependent hyperpolarization of arteriolar smooth muscle. / Huang, An; Sun, Dong; Jacobson, Azita; Carroll, Mairead A.; Falck, J R; Kaley, Gabor.

In: Circulation Research, Vol. 96, No. 3, 18.02.2005, p. 376-383.

Research output: Contribution to journalArticle

Huang, An ; Sun, Dong ; Jacobson, Azita ; Carroll, Mairead A. ; Falck, J R ; Kaley, Gabor. / Epoxyeicosatrienoic acids are released to mediate shear stress-dependent hyperpolarization of arteriolar smooth muscle. In: Circulation Research. 2005 ; Vol. 96, No. 3. pp. 376-383.
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AB - We hypothesized that shear stress stimulates the release of epoxyeicosatrienoic acids (EETs) from arteriolar endothelium, which directly hyperpolarize smooth muscle. To test this hypothesis, a perfusion system, consisting of two separate, serially connected chambers (A and B), was used. A donor vessel, isolated from gracilis muscle of female NO-deficient mice and rats, was cannulated in chamber A. In chamber B, an endothelium-denuded detector vessel isolated from mesentery of these animals was cannulated. In the presence of indomethacin, 5, 10, and 20 dyne/cm2 shear stress elicited dilation of donor vessels, followed by dilation of detector vessels. Changes in membrane potential of the detector vessel smooth muscle cells in response to the perfusate from 5 and 10 dyne/cm2 shear stress-stimulated donor vessels was also recorded (by ≈=-12 to -15 and -20 to -30 mV, respectively). Exposing detector vessels to 30 mmol/L KCl or pretreating them with iberiotoxin abolished their hyperpolarization and dilation to the flow of perfusate. Pretreatment of donor vessels with PPOH, an inhibitor of cytochrome P-450/epoxygenase, eliminated dilator responses in both donor and detector vessels, as well as the hyperpolarization of detector vessels. GC-MS analysis showed increasing release of EETs into the perfusate collected from 1, 5, and 10 dyne/cm2 shear stress-stimulated arterioles, which was abolished by PPOH. Thus, EETs, released from endothelial cells of donor vessels stimulated with shear stress, hyperpolarize smooth muscle of downstream detector vessels, confirming their identity as endothelium-derived hyperpolarizing factors and suggesting that gap junctional communication may not be necessary for shear stress-stimulated EDHF-mediated vasodilation.

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