Equilibration in Aqueous Methanol Increases the Resolution of Two-Dimensional Polyacrylamide Gels

Woodring E. Wright

doi:10.1080/01496398208055651

Equilibration in Aqueous Methanol Increases the Resolution of Two-Dimensional Polyacrylamide Gels

Woodring E. Wright

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Equilibrating cylindrical isoelcctrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.

Original language	English (US)
Pages (from-to)	1689-1696
Number of pages	8
Journal	Separation Science and Technology
Volume	17
Issue number	15
DOIs	https://doi.org/10.1080/01496398208055651
State	Published - Dec 1 1982

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)
Process Chemistry and Technology
Filtration and Separation

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10.1080/01496398208055651

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title = "Equilibration in Aqueous Methanol Increases the Resolution of Two-Dimensional Polyacrylamide Gels",

abstract = "Equilibrating cylindrical isoelcctrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.",

author = "Wright, {Woodring E.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health and the Muscular Dystrophy Association.",

year = "1982",

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N1 - Funding Information: This work was supported by grants from the National Institutes of Health and the Muscular Dystrophy Association.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - Equilibrating cylindrical isoelcctrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.

AB - Equilibrating cylindrical isoelcctrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.

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Equilibration in Aqueous Methanol Increases the Resolution of Two-Dimensional Polyacrylamide Gels

Abstract

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