Equilibration in Aqueous Methanol Increases the Resolution of Two-Dimensional Polyacrylamide Gels

Woodring E. Wright

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Equilibrating cylindrical isoelcctrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.

Original languageEnglish (US)
Pages (from-to)1689-1696
Number of pages8
JournalSeparation Science and Technology
Volume17
Issue number15
DOIs
StatePublished - Dec 1 1982

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ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)
  • Process Chemistry and Technology
  • Filtration and Separation

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