EQUILIBRATION IN AQUEOUS METHANOL INCREASES THE RESOLUTION OF TWO-DIMENSIONAL POLYACRYLAMIDE GELS.

Woodring E. Wright

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Equilibrating cylindrical isoelectrofocusing gels in 55% methanol rather than in aqueous buffer before starting the SDS dimension substantially increased the resolution obtained on 2-D gels. This effect may result from the in situ precipitation of focused proteins eliminating band diffusion prior to the second dimension.

Original languageEnglish (US)
Pages (from-to)1689-1696
Number of pages8
JournalSeparation Science and Technology
Volume17
Issue number15
StatePublished - Dec 1982

Fingerprint

Polyacrylates
Methanol
Gels
Buffers
Proteins
polyacrylamide gels

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Filtration and Separation
  • Process Chemistry and Technology
  • Chemistry(all)

Cite this

EQUILIBRATION IN AQUEOUS METHANOL INCREASES THE RESOLUTION OF TWO-DIMENSIONAL POLYACRYLAMIDE GELS. / Wright, Woodring E.

In: Separation Science and Technology, Vol. 17, No. 15, 12.1982, p. 1689-1696.

Research output: Contribution to journalArticle

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