Establishment and characterization of an Epstein-Barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient

Joan H. Schiller, Gerard Bittner, Lorraine F. Meisner, Terry D. Oberley, Diane Norback, Michael Schwabe, Connie R. Faltynek, Nancy Raab-Traub

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Hairy cell leukemia is a rare, B-cell malignancy uniquely sensitive to the antitumor effects of alpha and beta interferons (IFN). In order to further study the effects of IFN in this disease, we derived a cell line (HC1) from the peripheral blood mononuclear cells of a patient with hairy cell leukemia (HCL). Cells exhibited the typical morphological features of HCL, Including the characteristic cytoplasmic projections by light, transmission, and scanning electron microscopy. HC1 cells were of B-cell lineage, as evidenced by immunophenotypic analysis. Although originally TRAP positive, HC1 cells lost this biochemical marker following 3 months in culture. Monoclonality of the cell line was confirmed by a clonal karyotypic abnormality characteristic of B-cell malignancies, and the presence of a single, distinctive fused terminal EBV fragment. The cells formed colonies in soft agar and were tumorlgenic in irradiated nude mice. HC1 cells were sensitive to the antiproliferative effects of IFN-α and IFN-β, but only moderately sensitive to the growth inhibitory effects of IFN-γ. Incubating the cells in the presence of Type 1 IFN resulted in stabilization of cell numbers, without cellular proliferation or loss. Cell cycle analysis revealed that IFN-α resulted in a build-up of cells in the S phase of the cell cycle, suggesting a cytostatic effect of IFN on the growth of these cells. The HC1 cell line provides a model system which will be useful for in vitro studies of the biology and treatment of this disease.

Original languageEnglish (US)
Pages (from-to)399-407
Number of pages9
JournalLeukemia
Volume5
Issue number5
StatePublished - May 1991

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Hairy Cell Leukemia
Transformed Cell Line
Human Herpesvirus 4
Interferons
B-Lymphocytes
Cell Line
Cell Cycle
Interferon Type I
Scanning Transmission Electron Microscopy
Interferon-beta
Cytostatic Agents
Cell Lineage
Growth
S Phase
Interferon-alpha
Nude Mice
Agar
Blood Cells
Neoplasms
Cell Count

ASJC Scopus subject areas

  • Cancer Research
  • Hematology

Cite this

Schiller, J. H., Bittner, G., Meisner, L. F., Oberley, T. D., Norback, D., Schwabe, M., ... Raab-Traub, N. (1991). Establishment and characterization of an Epstein-Barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient. Leukemia, 5(5), 399-407.

Establishment and characterization of an Epstein-Barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient. / Schiller, Joan H.; Bittner, Gerard; Meisner, Lorraine F.; Oberley, Terry D.; Norback, Diane; Schwabe, Michael; Faltynek, Connie R.; Raab-Traub, Nancy.

In: Leukemia, Vol. 5, No. 5, 05.1991, p. 399-407.

Research output: Contribution to journalArticle

Schiller, JH, Bittner, G, Meisner, LF, Oberley, TD, Norback, D, Schwabe, M, Faltynek, CR & Raab-Traub, N 1991, 'Establishment and characterization of an Epstein-Barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient', Leukemia, vol. 5, no. 5, pp. 399-407.
Schiller, Joan H. ; Bittner, Gerard ; Meisner, Lorraine F. ; Oberley, Terry D. ; Norback, Diane ; Schwabe, Michael ; Faltynek, Connie R. ; Raab-Traub, Nancy. / Establishment and characterization of an Epstein-Barr virus spontaneously transformed lymphocytic cell line derived from a hairy cell leukemia patient. In: Leukemia. 1991 ; Vol. 5, No. 5. pp. 399-407.
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