Establishment of a monoclonal antibody for human LXRα: Detection of LXRα protein expression in human macrophages

Yuichiro Watanabe, Toshiya Tanaka, Yasutoshi Uchiyama, Tetsu Takeno, Akashi Izumi, Hisahiko Yamashita, Junko Kumakura, Hiroko Iwanari, Jiang Shu-Ying, Makoto Naito, David J. Mangelsdorf, Takao Hamakubo, Tatsuhiko Kodama

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Abstract

Liver X activated receptor alpha (LXRα) forms a functional dimeric nuclear receptor with RXR that regulates the metabolism of several important lipids, including cholesterol and bile acids. As compared with RXR, the LXRα protein level in the cell is low and the LXRα protein itself is very hard to detect. We have previously reported that the mRNA for LXRα is highly expressed in human cultured macrophages. In order to confirm the presence of the LXRα protein in the human macrophage, we have established a monoclonal antibody against LXRα, K-8607. The binding of mAb K-8607 to the human LXRα protein was confirmed by a wide variety of different techniques, including immunoblotting, immunohistochemistry, and electrophoretic mobility shift assay (EMSA). By immunoblotting with this antibody, the presence of native LXR protein in primary cultured human macrophage was demonstrated, as was its absence in human monocytes. This monoclonal anti-LXRα antibody should prove to be a useful tool in the analysis of the human LXRα protein.

Original languageEnglish (US)
Article number1
JournalNuclear Receptor
Volume1
DOIs
StatePublished - May 9 2003

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ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Watanabe, Y., Tanaka, T., Uchiyama, Y., Takeno, T., Izumi, A., Yamashita, H., Kumakura, J., Iwanari, H., Shu-Ying, J., Naito, M., Mangelsdorf, D. J., Hamakubo, T., & Kodama, T. (2003). Establishment of a monoclonal antibody for human LXRα: Detection of LXRα protein expression in human macrophages. Nuclear Receptor, 1, [1]. https://doi.org/10.1186/1478-1336-1-1