Eukaryotic Ribosome Assembly and Nuclear Export

Purnima Nerurkar, Martin Altvater, Stefan Gerhardy, Sabina Schütz, Ute Fischer, Christine Weirich, Vikram Govind Panse

Research output: Chapter in Book/Report/Conference proceedingConference contribution

43 Citations (Scopus)

Abstract

Accurate translation of the genetic code into functional polypeptides is key to cellular growth and proliferation. This essential process is carried out by the ribosome, a ribonucleoprotein complex of remarkable size and intricacy. Although the structure of the mature ribosome has provided insight into the mechanism of translation, our knowledge regarding the assembly, quality control, and intracellular targeting of this molecular machine is still emerging. Assembly of the eukaryotic ribosome begins in the nucleolus and requires more than 350 conserved assembly factors, which transiently associate with the preribosome at specific maturation stages. After accomplishing their tasks, early-acting. assembly factors are released, preparing preribosomes for nuclear export. Export competent preribosomal subunits are transported through nuclear pore complexes into the cytoplasm, where they undergo final maturation steps, which are closely connected to quality control, before engaging in translation. In this chapter, we focus on the final events that commit correctly assembled ribosomal subunits for translation.

Original languageEnglish (US)
Title of host publicationInternational Review of Cell and Molecular Biology, 2015
PublisherElsevier Inc.
Pages107-140
Number of pages34
Volume319
ISBN (Print)9780128022788
DOIs
StatePublished - Jan 1 2015

Publication series

NameInternational Review of Cell and Molecular Biology
Volume319
ISSN (Print)1937-6448

Fingerprint

Cell Nucleus Active Transport
Ribosomes
Quality Control
Genetic Code
Quality control
Nuclear Pore
Ribosome Subunits
Ribonucleoproteins
Translational Medical Research
Cytoplasm
Cell Proliferation
Peptides
Growth

Keywords

  • 40S subunits
  • 60S subunits
  • Biogenesis
  • Eukaryotic cells
  • Nuclear transport
  • Ribosome

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nerurkar, P., Altvater, M., Gerhardy, S., Schütz, S., Fischer, U., Weirich, C., & Panse, V. G. (2015). Eukaryotic Ribosome Assembly and Nuclear Export. In International Review of Cell and Molecular Biology, 2015 (Vol. 319, pp. 107-140). (International Review of Cell and Molecular Biology; Vol. 319). Elsevier Inc.. https://doi.org/10.1016/bs.ircmb.2015.07.002

Eukaryotic Ribosome Assembly and Nuclear Export. / Nerurkar, Purnima; Altvater, Martin; Gerhardy, Stefan; Schütz, Sabina; Fischer, Ute; Weirich, Christine; Panse, Vikram Govind.

International Review of Cell and Molecular Biology, 2015. Vol. 319 Elsevier Inc., 2015. p. 107-140 (International Review of Cell and Molecular Biology; Vol. 319).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Nerurkar, P, Altvater, M, Gerhardy, S, Schütz, S, Fischer, U, Weirich, C & Panse, VG 2015, Eukaryotic Ribosome Assembly and Nuclear Export. in International Review of Cell and Molecular Biology, 2015. vol. 319, International Review of Cell and Molecular Biology, vol. 319, Elsevier Inc., pp. 107-140. https://doi.org/10.1016/bs.ircmb.2015.07.002
Nerurkar P, Altvater M, Gerhardy S, Schütz S, Fischer U, Weirich C et al. Eukaryotic Ribosome Assembly and Nuclear Export. In International Review of Cell and Molecular Biology, 2015. Vol. 319. Elsevier Inc. 2015. p. 107-140. (International Review of Cell and Molecular Biology). https://doi.org/10.1016/bs.ircmb.2015.07.002
Nerurkar, Purnima ; Altvater, Martin ; Gerhardy, Stefan ; Schütz, Sabina ; Fischer, Ute ; Weirich, Christine ; Panse, Vikram Govind. / Eukaryotic Ribosome Assembly and Nuclear Export. International Review of Cell and Molecular Biology, 2015. Vol. 319 Elsevier Inc., 2015. pp. 107-140 (International Review of Cell and Molecular Biology).
@inproceedings{5d5a02239eb248968b0af8488a0c4a1b,
title = "Eukaryotic Ribosome Assembly and Nuclear Export",
abstract = "Accurate translation of the genetic code into functional polypeptides is key to cellular growth and proliferation. This essential process is carried out by the ribosome, a ribonucleoprotein complex of remarkable size and intricacy. Although the structure of the mature ribosome has provided insight into the mechanism of translation, our knowledge regarding the assembly, quality control, and intracellular targeting of this molecular machine is still emerging. Assembly of the eukaryotic ribosome begins in the nucleolus and requires more than 350 conserved assembly factors, which transiently associate with the preribosome at specific maturation stages. After accomplishing their tasks, early-acting. assembly factors are released, preparing preribosomes for nuclear export. Export competent preribosomal subunits are transported through nuclear pore complexes into the cytoplasm, where they undergo final maturation steps, which are closely connected to quality control, before engaging in translation. In this chapter, we focus on the final events that commit correctly assembled ribosomal subunits for translation.",
keywords = "40S subunits, 60S subunits, Biogenesis, Eukaryotic cells, Nuclear transport, Ribosome",
author = "Purnima Nerurkar and Martin Altvater and Stefan Gerhardy and Sabina Sch{\"u}tz and Ute Fischer and Christine Weirich and Panse, {Vikram Govind}",
year = "2015",
month = "1",
day = "1",
doi = "10.1016/bs.ircmb.2015.07.002",
language = "English (US)",
isbn = "9780128022788",
volume = "319",
series = "International Review of Cell and Molecular Biology",
publisher = "Elsevier Inc.",
pages = "107--140",
booktitle = "International Review of Cell and Molecular Biology, 2015",

}

TY - GEN

T1 - Eukaryotic Ribosome Assembly and Nuclear Export

AU - Nerurkar, Purnima

AU - Altvater, Martin

AU - Gerhardy, Stefan

AU - Schütz, Sabina

AU - Fischer, Ute

AU - Weirich, Christine

AU - Panse, Vikram Govind

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Accurate translation of the genetic code into functional polypeptides is key to cellular growth and proliferation. This essential process is carried out by the ribosome, a ribonucleoprotein complex of remarkable size and intricacy. Although the structure of the mature ribosome has provided insight into the mechanism of translation, our knowledge regarding the assembly, quality control, and intracellular targeting of this molecular machine is still emerging. Assembly of the eukaryotic ribosome begins in the nucleolus and requires more than 350 conserved assembly factors, which transiently associate with the preribosome at specific maturation stages. After accomplishing their tasks, early-acting. assembly factors are released, preparing preribosomes for nuclear export. Export competent preribosomal subunits are transported through nuclear pore complexes into the cytoplasm, where they undergo final maturation steps, which are closely connected to quality control, before engaging in translation. In this chapter, we focus on the final events that commit correctly assembled ribosomal subunits for translation.

AB - Accurate translation of the genetic code into functional polypeptides is key to cellular growth and proliferation. This essential process is carried out by the ribosome, a ribonucleoprotein complex of remarkable size and intricacy. Although the structure of the mature ribosome has provided insight into the mechanism of translation, our knowledge regarding the assembly, quality control, and intracellular targeting of this molecular machine is still emerging. Assembly of the eukaryotic ribosome begins in the nucleolus and requires more than 350 conserved assembly factors, which transiently associate with the preribosome at specific maturation stages. After accomplishing their tasks, early-acting. assembly factors are released, preparing preribosomes for nuclear export. Export competent preribosomal subunits are transported through nuclear pore complexes into the cytoplasm, where they undergo final maturation steps, which are closely connected to quality control, before engaging in translation. In this chapter, we focus on the final events that commit correctly assembled ribosomal subunits for translation.

KW - 40S subunits

KW - 60S subunits

KW - Biogenesis

KW - Eukaryotic cells

KW - Nuclear transport

KW - Ribosome

UR - http://www.scopus.com/inward/record.url?scp=84938631140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84938631140&partnerID=8YFLogxK

U2 - 10.1016/bs.ircmb.2015.07.002

DO - 10.1016/bs.ircmb.2015.07.002

M3 - Conference contribution

C2 - 26404467

AN - SCOPUS:84938631140

SN - 9780128022788

VL - 319

T3 - International Review of Cell and Molecular Biology

SP - 107

EP - 140

BT - International Review of Cell and Molecular Biology, 2015

PB - Elsevier Inc.

ER -