Evaluation of a method for study of kinetics of autologous apolipoprotein A-I

Gloria L Vega, H. Gylling, A. V. Nichols, Scott M Grundy

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Apolipoprotein A-I (apoA-I) participates in transport of plasma cholesterol. Concentrations of apoA-I depend on the balance between production and fractional clearance. To elucidate factors influencing apoA-I levels, accurate estimates of apoA-I turnover rates may be valuable. We describe a method for isolation of autologous apoA-I and its use in turnover studies. Free apoA-I was isolated from high density lipoproteins (HDL) by treatment with guanidine hydrochloride. This free apoA-I was radioiodinated with 131I and injected into eleven subjects simultaneously with HDL labeled with 125I. Plasma die-away curves of free apoA-I (131I) and HDL apoA-I (125I) were compared; fractional clearance rates averaged 0.256 ± 0.019 (SEM) and 0.254 ± 0.017 pools/day, respectively. Although slight differences between the two die-away curves were noted for some of the patients, the differences were relatively small; for the group as a whole, average fractional catabolic rates were not significantly different. Thus, by isolation of autologous apoA-I under the conditions described, free apoA-I seemingly provides a valid method for estimating apoA-I turnover.

Original languageEnglish (US)
Pages (from-to)867-875
Number of pages9
JournalJournal of lipid research
Volume32
Issue number5
StatePublished - Jan 1 1991

Keywords

  • guanidine hydrochloride
  • high density lipoprotein
  • lipoproteins
  • radioiodination
  • turnover

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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