Evaluation of two bioluminescence-measuring instruments, the turner design and lumac systems, for the rapid screening of urine specimens

W. D. Welch, L. Thompson, M. Layman, P. M. Southern

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Two bioluminescence-measuring instruments, the Turner Design and Lumac systems, were compared with a standard plate culture method for their ability to rapidly screen 400 urine specimens. For cultures with <1,000 CFU/ml the Turner Design, with old and new evaluation formulas, gave 6.5 and 50.6% false-positive results, respectively, versus 17.6% at ≥500 relative light units with the Lumac. For cultures which had >105 CFU/ml the Turner Design gave 39% (old formula) and 14% (new formula) false-negative results compared with 4% at <200 relative light units with the Lumac. The microorganisms most frequently isolated in the false-negative cultures from either system were gram-positive cocci. Predictive values for a positive test at >105 CFU/ml were 77.4% (old formula) and 35.7% (new formula) for the Turner Design versus only 50% for the Lumac at ≥500 relative light units. Predictive values for a negative test for both instruments were >88% at >105 CFU/ml. The Turner Design and Lumac systems were 4.0 and 3.7 times as expensive, respectively, as the plate culture method. Although both systems greatly reduce the time required to process urine specimens, their high costs as compared with that of plate culture, their failure to detect many specimens having >105 CFU of gram-positive cocci per ml, and the numerous false-positives reported by both instruments suggest that additional improvements in the systems are warranted.

Original languageEnglish (US)
Pages (from-to)1165-1170
Number of pages6
JournalJournal of clinical microbiology
Volume20
Issue number6
StatePublished - Dec 1 1984

ASJC Scopus subject areas

  • Microbiology (medical)

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