Evidence for involvement of HMGB1 protein in human DNA mismatch repair

Fenghua Yuan, Liya Gu, Shuangli Guo, Chunmei Wang, Guo Min Li

Research output: Contribution to journalArticle

94 Scopus citations

Abstract

Defects in human DNA mismatch repair predispose to cancer, but many components of the pathway have not been identified. We report here the identification and characterization of a novel component required for mismatch repair in human cells. A 30-kDa protein was purified to homogeneity by virtue of its ability to complement a depleted HeLa extract in repair of mismatched heteroduplexes. The complementing activity was identified as HMGB1 (the high mobility group box 1 protein), a non-histone chromatin protein that facilitates protein-protein interactions and recognizes DNA damage. Evidence is also presented that HMGB1 physically interacts with MutSα and is required at a step prior to the excision of mispaired nucleotide in mismatch repair.

Original languageEnglish (US)
Pages (from-to)20935-20940
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number20
DOIs
StatePublished - May 14 2004

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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