Abstract
Defects in human DNA mismatch repair predispose to cancer, but many components of the pathway have not been identified. We report here the identification and characterization of a novel component required for mismatch repair in human cells. A 30-kDa protein was purified to homogeneity by virtue of its ability to complement a depleted HeLa extract in repair of mismatched heteroduplexes. The complementing activity was identified as HMGB1 (the high mobility group box 1 protein), a non-histone chromatin protein that facilitates protein-protein interactions and recognizes DNA damage. Evidence is also presented that HMGB1 physically interacts with MutSα and is required at a step prior to the excision of mispaired nucleotide in mismatch repair.
Original language | English (US) |
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Pages (from-to) | 20935-20940 |
Number of pages | 6 |
Journal | Journal of Biological Chemistry |
Volume | 279 |
Issue number | 20 |
DOIs | |
State | Published - May 14 2004 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology