Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein βγ subunits is used for recognition of a subclass of effectors

Jamie K. Scott, Shan Fu Huang, Beechanahalli P. Gangadhar, Gary M. Samoriski, Peter Clapp, Robert A. Gross, Ronald Taussig, Alan V. Smrcka

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

To understand the requirements for binding to G protein βγ subunits, phage-displayed random peptide libraries were screened using immobilized biotinylated βγ as the target. Selected peptides were grouped into four different families based on their sequence characteristics. One group (group I) had a clear conserved motif that has significant homology to peptides derived from phospholipase C β (PLC β) and to a short motif in phosducin that binds to G protein β subunits. The other groups had weaker sequence homologies or no homology to the group I sequences. A synthetic peptide from the strongest consensus group blocked activation of PLC by G protein βγ subunits. The peptide did not block βγ-mediated inhibition of voltage-gated calcium channels and had little effect on βγ-mediated inhibition of Gs-stimulated type I adenylate cyclase. Competition experiments indicated that peptides from all four families bound to a single site on βγ. These peptides may bind to a protein-protein interaction 'hot spot' on the surface of βγ subunits that is used by a subclass of effectors.

Original languageEnglish (US)
Pages (from-to)767-776
Number of pages10
JournalEMBO Journal
Volume20
Issue number4
DOIs
StatePublished - Feb 15 2001

Fingerprint

Heterotrimeric GTP-Binding Proteins
Protein Subunits
Peptides
GTP-Binding Proteins
Proteins
Type C Phospholipases
Peptide Library
Bacteriophages
Calcium Channels
Sequence Homology
Adenylyl Cyclases
Chemical activation
Electric potential

Keywords

  • G protein βγ subunits
  • G protein effectors
  • Peptide
  • Phage display
  • Protein-protein interaction

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Scott, J. K., Huang, S. F., Gangadhar, B. P., Samoriski, G. M., Clapp, P., Gross, R. A., ... Smrcka, A. V. (2001). Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein βγ subunits is used for recognition of a subclass of effectors. EMBO Journal, 20(4), 767-776. https://doi.org/10.1093/emboj/20.4.767

Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein βγ subunits is used for recognition of a subclass of effectors. / Scott, Jamie K.; Huang, Shan Fu; Gangadhar, Beechanahalli P.; Samoriski, Gary M.; Clapp, Peter; Gross, Robert A.; Taussig, Ronald; Smrcka, Alan V.

In: EMBO Journal, Vol. 20, No. 4, 15.02.2001, p. 767-776.

Research output: Contribution to journalArticle

Scott, Jamie K. ; Huang, Shan Fu ; Gangadhar, Beechanahalli P. ; Samoriski, Gary M. ; Clapp, Peter ; Gross, Robert A. ; Taussig, Ronald ; Smrcka, Alan V. / Evidence that a protein-protein interaction 'hot spot' on heterotrimeric G protein βγ subunits is used for recognition of a subclass of effectors. In: EMBO Journal. 2001 ; Vol. 20, No. 4. pp. 767-776.
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