EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival

Zachary R. Gallegos, Patrick Taus, Zane A. Gibbs, Kathleen McGlynn, Nicholas C. Gomez, Ian Davis, Angelique W Whitehurst

Research output: Contribution to journalArticle

Abstract

Ewing sarcoma is characterized by a pathognomonic chromosomal translocation that generates the EWSR1-FLI1 chimeric transcription factor. The transcriptional targets of EWSR1-FLI1 that are essential for tumorigenicity are incompletely defined. Here, we found that EWSR1-FLI1 modulates the expression of cancer/testis (CT) antigen genes, whose expression is biased to the testes but is also activated in cancer. Among these CT antigens, fetal and adult testis expressed 1 (FATE1) is most robustly induced. EWSR1-FLI1 associates with the GGAA repeats in the proximal promoter of FATE1, which exhibits accessible chromatin exclusively in mesenchymal progenitor cells (MPCs) and Ewing sarcoma cells. Expression of EWSR1-FLI1 in non-Ewing sarcoma cells and in MPCs enhances FATE1 mRNA and protein expression. Conversely, depletion of EWSR1-FLI1 in Ewing sarcoma cells leads to a loss of FATE1 expression. Importantly, we found that FATE1 is required for survival and anchorage-independent growth in Ewing sarcoma cells via attenuating the accumulation of BNIP3L, a BH3-only protein that is toxic when stabilized. This action appears to be mediated by the E3 ligase RNF183. We propose that engaging FATE1 function can permit the bypass of cell death mechanisms that would otherwise inhibit tumor progression.

Original languageEnglish (US)
JournalMolecular and cellular biology
Volume39
Issue number14
DOIs
StatePublished - Jul 15 2019

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Ewing's Sarcoma
Testicular Neoplasms
Testis
Antigens
Mesenchymal Stromal Cells
Genetic Translocation
Ubiquitin-Protein Ligases
Poisons
Sarcoma
Chromatin
Neoplasms
Proteins
Cell Death
Transcription Factors
Gene Expression
Messenger RNA
Growth

Keywords

  • BNIP3L
  • cancer/testis antigen
  • CT antigen
  • Ewing sarcoma
  • EWSR1-FLI1
  • FATE1
  • RNF183

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival. / Gallegos, Zachary R.; Taus, Patrick; Gibbs, Zane A.; McGlynn, Kathleen; Gomez, Nicholas C.; Davis, Ian; Whitehurst, Angelique W.

In: Molecular and cellular biology, Vol. 39, No. 14, 15.07.2019.

Research output: Contribution to journalArticle

Gallegos, Zachary R. ; Taus, Patrick ; Gibbs, Zane A. ; McGlynn, Kathleen ; Gomez, Nicholas C. ; Davis, Ian ; Whitehurst, Angelique W. / EWSR1-FLI1 Activation of the Cancer/Testis Antigen FATE1 Promotes Ewing Sarcoma Survival. In: Molecular and cellular biology. 2019 ; Vol. 39, No. 14.
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