Excision of thymine dimers by proteolytic and amber fragments of E. coli DNA polymerase I

Errol C. Friedberg, I. R. Lehman

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Abstract

Excision of thymine dimers from specifically incised ultraviolet irradiated DNA by E. coli DNA polymerase I is stimulated by concurrent DNA synthesis. The 36,000 molecular-weight "small fragment" obtained by limited proteolysis of DNA polymerase I, which retains only the 5′ → 3′ exonuclease activity, also excises thymine dimers, but at one-tenth the rate of the intact enzyme. However, the rate of excision is increased by addition of the "large" 76,000-molecular weight fragment. With the further addition of the 4 deoxynucleoside triphosphates, permitting DNA synthesis to occur, excision approaches rates observed with the intact enzyme. The same result was obtained with a fragment of DNA polymerase I with 5′ → 3′ exonuclease activity that is present uniquely in polymerase I amber mutants.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number1
DOIs
StatePublished - May 7 1974

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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