Excision of thymine dimers by proteolytic and amber fragments of E. coli DNA polymerase I

Errol C. Friedberg, I. R. Lehman

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Excision of thymine dimers from specifically incised ultraviolet irradiated DNA by E. coli DNA polymerase I is stimulated by concurrent DNA synthesis. The 36,000 molecular-weight "small fragment" obtained by limited proteolysis of DNA polymerase I, which retains only the 5′ → 3′ exonuclease activity, also excises thymine dimers, but at one-tenth the rate of the intact enzyme. However, the rate of excision is increased by addition of the "large" 76,000-molecular weight fragment. With the further addition of the 4 deoxynucleoside triphosphates, permitting DNA synthesis to occur, excision approaches rates observed with the intact enzyme. The same result was obtained with a fragment of DNA polymerase I with 5′ → 3′ exonuclease activity that is present uniquely in polymerase I amber mutants.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume58
Issue number1
DOIs
StatePublished - May 7 1974

Fingerprint

Amber
Pyrimidine Dimers
DNA Polymerase I
Escherichia coli
Exonucleases
DNA
Molecular Weight
Molecular weight
Proteolysis
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Excision of thymine dimers by proteolytic and amber fragments of E. coli DNA polymerase I. / Friedberg, Errol C.; Lehman, I. R.

In: Biochemical and Biophysical Research Communications, Vol. 58, No. 1, 07.05.1974, p. 132-139.

Research output: Contribution to journalArticle

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