Experimental model for heart failure in rats - Induction and diagnosis

V. Zaha, J. Grohmann, H. Göbel, A. Geibel, F. Beyersdorf, Torsten Doenst

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: Clinical heart failure is generally preceded by hypertrophy. Many animal models (e.g. toxic heart failure models) do not consider this hypertrophy. We set out to develop a heart failure model in rats by inducing pressure-overload hypertrophy. Methods: We induced coarctation of the aortic arch with a tantalum clip (0.35 mm internal diameter) In 3-week-old rats (n= 17). Starting at seven weeks postoperatively, we measured ejection fraction (EF), fractional shortening (FS), end-systolic (LVESD) and end-diastolic (LVEDD) left ventricular dimensions by echocardiography each week. Heart, lung, and liver specimens were analyzed histopathologically at least eleven weeks after the operation. Results: Contractile function was significantly decreased in hearts from animals with aortic banding (EF: 45±5% vs. 73±5%, p<0.01; FS: 20±3% vs. 35±5%, p < 0.01). At the same time, left ventricles were dilated (LVEDD: 9.1±0.6mm vs. 7.4±0.5mm; LVESD: 7.3±0.6mm vs. 4.8± 0.4 mm, p < 0.01). These observations were associated with clinical and histopathological changes characteristic for chronic left heart failure. Conclusion: Placing a tantalum clip around the aortic arch in 3-week-old rats consistently induces left ventricular decrease in contractile function and dilatation after eleven weeks.

Original languageEnglish (US)
Pages (from-to)211-215
Number of pages5
JournalThoracic and Cardiovascular Surgeon
Volume51
Issue number4
DOIs
StatePublished - Aug 1 2003

Fingerprint

Theoretical Models
Heart Failure
Hypertrophy
Thoracic Aorta
Surgical Instruments
Poisons
Heart Ventricles
Echocardiography
Dilatation
Animal Models
Pressure
Lung
Liver

Keywords

  • Congestive
  • Echocardiography
  • Heart failure
  • Hypertrophy
  • Myocardium

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Experimental model for heart failure in rats - Induction and diagnosis. / Zaha, V.; Grohmann, J.; Göbel, H.; Geibel, A.; Beyersdorf, F.; Doenst, Torsten.

In: Thoracic and Cardiovascular Surgeon, Vol. 51, No. 4, 01.08.2003, p. 211-215.

Research output: Contribution to journalArticle

Zaha, V, Grohmann, J, Göbel, H, Geibel, A, Beyersdorf, F & Doenst, T 2003, 'Experimental model for heart failure in rats - Induction and diagnosis', Thoracic and Cardiovascular Surgeon, vol. 51, no. 4, pp. 211-215. https://doi.org/10.1055/s-2003-42264
Zaha, V. ; Grohmann, J. ; Göbel, H. ; Geibel, A. ; Beyersdorf, F. ; Doenst, Torsten. / Experimental model for heart failure in rats - Induction and diagnosis. In: Thoracic and Cardiovascular Surgeon. 2003 ; Vol. 51, No. 4. pp. 211-215.
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