Experimental murine cytomegalovirus infection of ocular structures

J. F. Bale, M. E. O'Neil, R. N. Hogan, E. R. Kern

Research output: Contribution to journalArticle

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Abstract

To study experimental cytomegalovirus (CMV) infection of ocular structures, 3-week-old Swiss-Webster mice were inoculated intraperitoneally with a 0% to 20% lethal inoculum of murine CMV (MCMV). Murine cytomegalovirus was recovered from homogenates of eye tissues on days 3, 5, and 7 after inoculation. Peak virus titers, mean of 2.93 ± 0.67 log plaque-forming units of MCMV per gram of ocular tissue occurred on day 5. Urine cytomegalovirus was recovered from explant cultures of eye and optic nerve 14, 21, 60, 90, and 120 days after MCMV inoculation. Murine cytomegalovirus also persisted in intraocular fluids for as long as 90 days. Murine cytomegalovirus infection was confirmed by immunofluorescence antibody staining and transmission electron microscopy. These experiments indicate that MCMV infects the ocular tissues of mice during systemic MCMV infection, and persists in ocular tissues for as long as 120 days after infection. These studies may be directly relevant to ocular disorders that occur during acquired CMV infections of humans, and suggest that ocular tissues may be a site of CMV persistence or latency.

Original languageEnglish (US)
Pages (from-to)1214-1219
Number of pages6
JournalArchives of Ophthalmology
Volume102
Issue number8
StatePublished - 1984

Fingerprint

Muromegalovirus
Cytomegalovirus Infections
Cytomegalovirus
Aqueous Humor
Optic Nerve
Infection
Viral Load
Transmission Electron Microscopy
Fluorescent Antibody Technique
Urine
Staining and Labeling
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Bale, J. F., O'Neil, M. E., Hogan, R. N., & Kern, E. R. (1984). Experimental murine cytomegalovirus infection of ocular structures. Archives of Ophthalmology, 102(8), 1214-1219.

Experimental murine cytomegalovirus infection of ocular structures. / Bale, J. F.; O'Neil, M. E.; Hogan, R. N.; Kern, E. R.

In: Archives of Ophthalmology, Vol. 102, No. 8, 1984, p. 1214-1219.

Research output: Contribution to journalArticle

Bale, JF, O'Neil, ME, Hogan, RN & Kern, ER 1984, 'Experimental murine cytomegalovirus infection of ocular structures', Archives of Ophthalmology, vol. 102, no. 8, pp. 1214-1219.
Bale, J. F. ; O'Neil, M. E. ; Hogan, R. N. ; Kern, E. R. / Experimental murine cytomegalovirus infection of ocular structures. In: Archives of Ophthalmology. 1984 ; Vol. 102, No. 8. pp. 1214-1219.
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