Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle

Orkun Tan, Turkan Ornek, Ahmed Fadiel, Kelley S. Carrick, Aydin Arici, Kevin Doody, Bruce R. Carr, Frederick Naftolin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. Design: Experimental laboratory study. Setting: University medical centers. Patient(s): Reproductive-age women. Intervention(s): A total of 36 samples of normal early, mid-, and late proliferative- and secretory-phase endometrium were studied for immunoreactive total ezrin (ir-T-ezrin) and phospho-ezrin (ir-p-ezrin) expression by histology, immunohistochemistry, and Western blotting. Main Outcome Measure(s): Total ezrin and phospho-ezrin expressions through the normal endometrial cycle. Result(s): Throughout the cycle ir-T-ezrin is present in the epithelium. The intensity and localization of both ir-ezrin and ir-p-ezrin vary greatly throughout the cycle. The main findings include the following: lateral localization of ir-ezrin/ir-p-ezrin in association with membrane specializations; dense staining around secretory vacuoles (secretory phase); dense staining of the apical surfaces, including microvilli and pinopodes of epithelial cells, especially during the mid- to late secretory phases; and the presence of ezrin in the glandular secretions. Immunoreactive total ezrin and ir-p-ezrin were not expressed by stromal fibroblasts. Conclusion(s): Ezrin is a prominent protein in the cycling endometrium. The most striking findings were the gravitation of ir-ezrin/ir-p-ezrin to the periphery of secretory vacuoles, localization on apical surfaces of the luminal epithelium, dense ezrin staining in secretory-phase epithelial cell plumes, and the presence of ir-ezrin/ir-p-ezrin in secretory-phase luminal secretions. These findings may have functional implications, especially for implantation biology.

Original languageEnglish (US)
JournalFertility and Sterility
Volume97
Issue number1
DOIs
StatePublished - Jan 2012

Fingerprint

Menstrual Cycle
Cytoskeleton
Membrane Proteins
ezrin
Staining and Labeling
Endometrium
Vacuoles
Epithelium
Epithelial Cells

Keywords

  • endometrium
  • Ezrin
  • histology
  • implantation
  • phospho-ezrin
  • pinopodes

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle. / Tan, Orkun; Ornek, Turkan; Fadiel, Ahmed; Carrick, Kelley S.; Arici, Aydin; Doody, Kevin; Carr, Bruce R.; Naftolin, Frederick.

In: Fertility and Sterility, Vol. 97, No. 1, 01.2012.

Research output: Contribution to journalArticle

Tan, Orkun ; Ornek, Turkan ; Fadiel, Ahmed ; Carrick, Kelley S. ; Arici, Aydin ; Doody, Kevin ; Carr, Bruce R. ; Naftolin, Frederick. / Expression and activation of the membrane-cytoskeleton protein ezrin during the normal endometrial cycle. In: Fertility and Sterility. 2012 ; Vol. 97, No. 1.
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abstract = "Objective: To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. Design: Experimental laboratory study. Setting: University medical centers. Patient(s): Reproductive-age women. Intervention(s): A total of 36 samples of normal early, mid-, and late proliferative- and secretory-phase endometrium were studied for immunoreactive total ezrin (ir-T-ezrin) and phospho-ezrin (ir-p-ezrin) expression by histology, immunohistochemistry, and Western blotting. Main Outcome Measure(s): Total ezrin and phospho-ezrin expressions through the normal endometrial cycle. Result(s): Throughout the cycle ir-T-ezrin is present in the epithelium. The intensity and localization of both ir-ezrin and ir-p-ezrin vary greatly throughout the cycle. The main findings include the following: lateral localization of ir-ezrin/ir-p-ezrin in association with membrane specializations; dense staining around secretory vacuoles (secretory phase); dense staining of the apical surfaces, including microvilli and pinopodes of epithelial cells, especially during the mid- to late secretory phases; and the presence of ezrin in the glandular secretions. Immunoreactive total ezrin and ir-p-ezrin were not expressed by stromal fibroblasts. Conclusion(s): Ezrin is a prominent protein in the cycling endometrium. The most striking findings were the gravitation of ir-ezrin/ir-p-ezrin to the periphery of secretory vacuoles, localization on apical surfaces of the luminal epithelium, dense ezrin staining in secretory-phase epithelial cell plumes, and the presence of ir-ezrin/ir-p-ezrin in secretory-phase luminal secretions. These findings may have functional implications, especially for implantation biology.",
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AU - Tan, Orkun

AU - Ornek, Turkan

AU - Fadiel, Ahmed

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AU - Arici, Aydin

AU - Doody, Kevin

AU - Carr, Bruce R.

AU - Naftolin, Frederick

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AB - Objective: To examine total ezrin expression (ezrin and phospho-ezrin) through the normal endometrial cycle and to correlate ezrin activation and localization with cytologic changes. Design: Experimental laboratory study. Setting: University medical centers. Patient(s): Reproductive-age women. Intervention(s): A total of 36 samples of normal early, mid-, and late proliferative- and secretory-phase endometrium were studied for immunoreactive total ezrin (ir-T-ezrin) and phospho-ezrin (ir-p-ezrin) expression by histology, immunohistochemistry, and Western blotting. Main Outcome Measure(s): Total ezrin and phospho-ezrin expressions through the normal endometrial cycle. Result(s): Throughout the cycle ir-T-ezrin is present in the epithelium. The intensity and localization of both ir-ezrin and ir-p-ezrin vary greatly throughout the cycle. The main findings include the following: lateral localization of ir-ezrin/ir-p-ezrin in association with membrane specializations; dense staining around secretory vacuoles (secretory phase); dense staining of the apical surfaces, including microvilli and pinopodes of epithelial cells, especially during the mid- to late secretory phases; and the presence of ezrin in the glandular secretions. Immunoreactive total ezrin and ir-p-ezrin were not expressed by stromal fibroblasts. Conclusion(s): Ezrin is a prominent protein in the cycling endometrium. The most striking findings were the gravitation of ir-ezrin/ir-p-ezrin to the periphery of secretory vacuoles, localization on apical surfaces of the luminal epithelium, dense ezrin staining in secretory-phase epithelial cell plumes, and the presence of ir-ezrin/ir-p-ezrin in secretory-phase luminal secretions. These findings may have functional implications, especially for implantation biology.

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KW - histology

KW - implantation

KW - phospho-ezrin

KW - pinopodes

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