Expression of acidic and basic fibroblast growth factors in the substantia nigra of rat, monkey, and human

Andrew J. Bean, Robert Elde, Yihai Cao, Cornelia Oellig, Carol Tamminga, Menek Goldstein, Ralf F. Pettersson, Tomas Hökfelt

Research output: Contribution to journalArticle

132 Scopus citations

Abstract

The distribution of acidic (aFGF) and basic (bFGF) fibroblast growth factor mRNA and protein were examined in mesencephalon by immunohistochemistry, immunoblot analysis, in situ hybridization histochemistry, and RNA analysis. Coexistence of aFGF or bFGF with tyrosine hydroxylase protein in nigral cells was observed with immunohistochemistry. Both aFGF and bFGF mRNAs were found in the substantia nigra. Unilateral 6-hydroxydopamine lesions of nigrostriatal neurons resulted in a loss of aFGF and tyrosine hydroxylase [L-tyrosine, tetrahydropteridine: oxygen oxidoreductase (3-hydroxylating), EC 1.14.16.2] mRNA-positive neurons on the lesioned side. The distribution of aFGF mRNA in monkey brain was similar to that seen in the rat. RNA and immunoblot analysis confirmed the presence of both aFGF and bFGF mRNAs and proteins in the substantia nigra of rat, monkey, and human.

Original languageEnglish (US)
Pages (from-to)10237-10241
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number22
DOIs
StatePublished - 1991

Keywords

  • Coexistence
  • Dopamine
  • Neurotrophic factor
  • Tyrosine hydroxylase
  • mRNA

ASJC Scopus subject areas

  • General

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