Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity

José C. Crispín, Brendan T. Keenan, Michele D. Finnell, Bonnie L. Bermas, Peter Schur, Elena Massarotti, Elizabeth W. Karlson, Lisa M. Fitzgerald, Sukran Ergin, Vasileios C. Kyttaris, George C. Tsokos, Karen H. Costenbader

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Abstract

Objective. To quantify the expression of CD44 and variant isoforms CD44v3 and CD44v6 on T cells from patients with systemic lupus erythematosus (SLE), and to assess correlations of the level of expression of these molecules with disease manifestations. Methods. Information on clinical and demographic characteristics was collected, and blood samples were obtained from 72 patients with SLE and 32 healthy control subjects matched to the patients by sex, race, and age. Expression of CD44 and variants CD44v3 and v6 on T cell subsets was determined by flow cytometry, and Pearson's correlations of their expression levels with clinical variables, SLE Disease Activity Index (SLEDAI) scores, and presence of lupus nephritis were determined. Wilcoxon's rank sum tests and conditional multivariable regression analyses were applied to identify differences in the expression of CD44 between patients with SLE and healthy controls. Results. Expression of CD44 was higher on CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Expression of CD44v3 and CD44v6 was also higher on total T cells and CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Cell surface levels of CD44v3 on total T cells, CD4+ T cells, and CD8+ T cells as well as cell surface expression of CD44v6 on total T cells and CD4+ T cells were correlated with the SLEDAI score (P < 0.05). The presence of lupus nephritis was associated with the expression of CD44v6 on total T cells, CD4+ T cells, and CD4-CD8- T cells (P < 0.05). Positivity for anti-double-stranded DNA antibodies was associated with the expression levels of CD44v6 on T cells (P < 0.05). Conclusion. These results indicate that expression levels of CD44v3 and CD44v6 on T cells may represent useful biomarkers of SLE activity.

Original languageEnglish (US)
Pages (from-to)1431-1437
Number of pages7
JournalArthritis and Rheumatism
Volume62
Issue number5
DOIs
StatePublished - May 1 2010

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Systemic Lupus Erythematosus
Protein Isoforms
T-Lymphocytes
Lupus Nephritis
Nonparametric Statistics
T-Lymphocyte Subsets
Healthy Volunteers
Flow Cytometry
Biomarkers
Regression Analysis
Demography

ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity. / Crispín, José C.; Keenan, Brendan T.; Finnell, Michele D.; Bermas, Bonnie L.; Schur, Peter; Massarotti, Elena; Karlson, Elizabeth W.; Fitzgerald, Lisa M.; Ergin, Sukran; Kyttaris, Vasileios C.; Tsokos, George C.; Costenbader, Karen H.

In: Arthritis and Rheumatism, Vol. 62, No. 5, 01.05.2010, p. 1431-1437.

Research output: Contribution to journalArticle

Crispín, JC, Keenan, BT, Finnell, MD, Bermas, BL, Schur, P, Massarotti, E, Karlson, EW, Fitzgerald, LM, Ergin, S, Kyttaris, VC, Tsokos, GC & Costenbader, KH 2010, 'Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity', Arthritis and Rheumatism, vol. 62, no. 5, pp. 1431-1437. https://doi.org/10.1002/art.27385
Crispín, José C. ; Keenan, Brendan T. ; Finnell, Michele D. ; Bermas, Bonnie L. ; Schur, Peter ; Massarotti, Elena ; Karlson, Elizabeth W. ; Fitzgerald, Lisa M. ; Ergin, Sukran ; Kyttaris, Vasileios C. ; Tsokos, George C. ; Costenbader, Karen H. / Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity. In: Arthritis and Rheumatism. 2010 ; Vol. 62, No. 5. pp. 1431-1437.
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abstract = "Objective. To quantify the expression of CD44 and variant isoforms CD44v3 and CD44v6 on T cells from patients with systemic lupus erythematosus (SLE), and to assess correlations of the level of expression of these molecules with disease manifestations. Methods. Information on clinical and demographic characteristics was collected, and blood samples were obtained from 72 patients with SLE and 32 healthy control subjects matched to the patients by sex, race, and age. Expression of CD44 and variants CD44v3 and v6 on T cell subsets was determined by flow cytometry, and Pearson's correlations of their expression levels with clinical variables, SLE Disease Activity Index (SLEDAI) scores, and presence of lupus nephritis were determined. Wilcoxon's rank sum tests and conditional multivariable regression analyses were applied to identify differences in the expression of CD44 between patients with SLE and healthy controls. Results. Expression of CD44 was higher on CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Expression of CD44v3 and CD44v6 was also higher on total T cells and CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Cell surface levels of CD44v3 on total T cells, CD4+ T cells, and CD8+ T cells as well as cell surface expression of CD44v6 on total T cells and CD4+ T cells were correlated with the SLEDAI score (P < 0.05). The presence of lupus nephritis was associated with the expression of CD44v6 on total T cells, CD4+ T cells, and CD4-CD8- T cells (P < 0.05). Positivity for anti-double-stranded DNA antibodies was associated with the expression levels of CD44v6 on T cells (P < 0.05). Conclusion. These results indicate that expression levels of CD44v3 and CD44v6 on T cells may represent useful biomarkers of SLE activity.",
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T1 - Expression of CD44 variant isoforms CD44v3 and CD44v6 is increased on T cells from patients with systemic lupus erythematosus and is correlated with disease activity

AU - Crispín, José C.

AU - Keenan, Brendan T.

AU - Finnell, Michele D.

AU - Bermas, Bonnie L.

AU - Schur, Peter

AU - Massarotti, Elena

AU - Karlson, Elizabeth W.

AU - Fitzgerald, Lisa M.

AU - Ergin, Sukran

AU - Kyttaris, Vasileios C.

AU - Tsokos, George C.

AU - Costenbader, Karen H.

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N2 - Objective. To quantify the expression of CD44 and variant isoforms CD44v3 and CD44v6 on T cells from patients with systemic lupus erythematosus (SLE), and to assess correlations of the level of expression of these molecules with disease manifestations. Methods. Information on clinical and demographic characteristics was collected, and blood samples were obtained from 72 patients with SLE and 32 healthy control subjects matched to the patients by sex, race, and age. Expression of CD44 and variants CD44v3 and v6 on T cell subsets was determined by flow cytometry, and Pearson's correlations of their expression levels with clinical variables, SLE Disease Activity Index (SLEDAI) scores, and presence of lupus nephritis were determined. Wilcoxon's rank sum tests and conditional multivariable regression analyses were applied to identify differences in the expression of CD44 between patients with SLE and healthy controls. Results. Expression of CD44 was higher on CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Expression of CD44v3 and CD44v6 was also higher on total T cells and CD4+ and CD8+ T cells from SLE patients compared with controls (P ≤ 0.03). Cell surface levels of CD44v3 on total T cells, CD4+ T cells, and CD8+ T cells as well as cell surface expression of CD44v6 on total T cells and CD4+ T cells were correlated with the SLEDAI score (P < 0.05). The presence of lupus nephritis was associated with the expression of CD44v6 on total T cells, CD4+ T cells, and CD4-CD8- T cells (P < 0.05). Positivity for anti-double-stranded DNA antibodies was associated with the expression levels of CD44v6 on T cells (P < 0.05). Conclusion. These results indicate that expression levels of CD44v3 and CD44v6 on T cells may represent useful biomarkers of SLE activity.

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