Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes

Robert S. Goodenow, Iwona Stroynowski, Minnie McMillan, Margery Nicolson, Kurt Eakle, Beverly Taylor Sher, Norman Davidson, Leroy Hood

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Mouse L cells transformed with the cloned class I genes of the major histocompatibility complex of the mouse express transplantation antigens with serological determinants of the donor haplotype. However, transformation with the truncated subclones of a BALB/c H-2Ld gene containing the exons encoding the external domains also leads to the production of cells which express complete cell-surface molecules. Moreover, full-length products of the foreign haplotype, as judged by serological and biochemical criteria, are generated independently of the use of carrier DNA in transformation. However, the frequency of productive transformation is substantially less than that obtained with a complete gene. The most plausible explanation for these phenomena involves homologous recombination between host chromosomal and donor class I sequences.

Original languageEnglish (US)
Pages (from-to)388-394
Number of pages7
JournalNature
Volume301
Issue number5899
DOIs
StatePublished - 1983

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Histocompatibility Antigens
Haplotypes
Genes
MHC Class I Genes
Homologous Recombination
Major Histocompatibility Complex
Exons
DNA

ASJC Scopus subject areas

  • General

Cite this

Goodenow, R. S., Stroynowski, I., McMillan, M., Nicolson, M., Eakle, K., Taylor Sher, B., ... Hood, L. (1983). Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes. Nature, 301(5899), 388-394. https://doi.org/10.1038/301388a0

Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes. / Goodenow, Robert S.; Stroynowski, Iwona; McMillan, Minnie; Nicolson, Margery; Eakle, Kurt; Taylor Sher, Beverly; Davidson, Norman; Hood, Leroy.

In: Nature, Vol. 301, No. 5899, 1983, p. 388-394.

Research output: Contribution to journalArticle

Goodenow, RS, Stroynowski, I, McMillan, M, Nicolson, M, Eakle, K, Taylor Sher, B, Davidson, N & Hood, L 1983, 'Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes', Nature, vol. 301, no. 5899, pp. 388-394. https://doi.org/10.1038/301388a0
Goodenow, Robert S. ; Stroynowski, Iwona ; McMillan, Minnie ; Nicolson, Margery ; Eakle, Kurt ; Taylor Sher, Beverly ; Davidson, Norman ; Hood, Leroy. / Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes. In: Nature. 1983 ; Vol. 301, No. 5899. pp. 388-394.
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