Expression of complete transplantation antigens by mammalian cells transformed with truncated class i genes

Robert S. Goodenow, Iwona Stroynowski, Minnie McMillan, Margery Nicolson, Kurt Eakle, Beverly Taylor Sher, Norman Davidson, Leroy Hood

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Mouse L cells transformed with the cloned class I genes of the major histocompatibility complex of the mouse express transplantation antigens with serological determinants of the donor haplotype. However, transformation with the truncated subclones of a BALB/c H-2Ld gene containing the exons encoding the external domains also leads to the production of cells which express complete cell-surface molecules. Moreover, full-length products of the foreign haplotype, as judged by serological and biochemical criteria, are generated independently of the use of carrier DNA in transformation. However, the frequency of productive transformation is substantially less than that obtained with a complete gene. The most plausible explanation for these phenomena involves homologous recombination between host chromosomal and donor class I sequences.

Original languageEnglish (US)
Pages (from-to)388-394
Number of pages7
JournalNature
Volume301
Issue number5899
DOIs
StatePublished - Dec 1 1983

ASJC Scopus subject areas

  • General

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